NOX2 Antibody (YA6330)
(Synonyms: CYBB; NOX2; Cytochrome b-245 heavy chain; CGD91-phox; Cytochrome b; 558; subunit beta; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2Neutrophil cytochrome b 91 kDa polypeptide; Superoxide-generating NADPH oxidase heavy chain subunit; gp91-1; gp91-phox; p22 phagocyte B-cytochrome)NOX2 Antibody (YA6330) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NOX2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IP, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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IP
IP: Immunoprecipitation
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|---|---|---|---|
| Dilution Ratio | 1:1000-5000 | 1:5000-20000 | 1:50-200 |
Product Details
NOX2 Antibody (YA6330) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NOX2.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 65 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 65 kDa
Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
NOX2 (NADPH oxidase 2) is a catalytic member of the NADPH oxidase family that is specialized for reactive oxygen species (ROS) generation and serves as a central component of phagocyte antimicrobial defense and redox signaling pathways[1][2]. NOX2 activation requires assembly of membrane and cytosolic regulatory subunits, enabling superoxide production at the plasma membrane or phagosomal membrane during immune responses[2]. Mechanistically, NOX2-derived ROS contribute not only to pathogen killing but also to immune regulation, antigen presentation, and maintenance of self-tolerance, linking oxidative signaling to both innate and adaptive immunity[3][4]. Dysregulated NOX2 activity has been implicated in multiple inflammatory and oxidative stress-associated disease models, while genetic deficiency of NOX2 causes chronic granulomatous disease characterized by impaired microbial killing and recurrent infections[3][5]. In disease models, NOX2-derived ROS have been associated with inflammation, fibrosis, and cardiovascular pathology, highlighting the broad biological relevance of this isoform[5][6]. Compared with related NOX isoforms, NOX2 is predominantly expressed in phagocytic cells, whereas NOX1, NOX3, NOX4, and NOX5 are primarily expressed in non-phagocytic tissues, indicating distinct physiological functions and cellular contexts[7]. For experimental applications, NOX inhibitors and NOX2-targeted inhibitory peptides such as NOX2ds-tat have been used to investigate NOX2-dependent signaling; however, limited isoform selectivity remains an important consideration when interpreting pharmacological studies[8].
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Subcellular Localization
Cell membrane; Multi-pass membrane protein
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Expression
Tissue_specificity:Protein levels were detected in neutrophils. -
Subunit
Component of the phagocyte NADPH oxidase core complex/cytochrome b558 complex, composed of CYBB (heavy chain (beta)) and CYBA (light chain (alpha)) (PubMed:3600769, PubMed:36241643, PubMed:36413210). Component of the phagocyte NADPH oxidase complex composed of an obligatory core heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic regulatory subunits NCF1/p47-phox, NCF2/p67-phox, NCF4/p40-phox and the small GTPase RAC1 or RAC2 (PubMed:15338276, PubMed:19028840, PubMed:38355798). Interacts with NCF1 (phosphorylated form) (PubMed:19028840, PubMed:9224653). Interacts with NCF2; the interaction is enhanced in the presence of GBP7 (PubMed:19028840, PubMed:38355798). Interacts with RAC2 (PubMed:19028840). Interacts with RAC1 (PubMed:38355798). Interacts with calprotectin (S100A8/9) (PubMed:22808130). Interacts with NRROS; the interaction is direct and impairs formation of a stable NADPH oxidase complex (By similarity). Interacts with CYBC1; CYBC1 may act as a chaperone stabilizing Cytochrome b-245 heterodimer (PubMed:28351984). The CYBA-CYBB complex interacts with GBP7 (By similarity)
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SwissProt ID
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Synonyms
CYBB; NOX2; Cytochrome b-245 heavy chain; CGD91-phox; Cytochrome b; 558; subunit beta; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2Neutrophil cytochrome b 91 kDa polypeptide; Superoxide-generating NADPH oxidase heavy chain subunit; gp91-1; gp91-phox; p22 phagocyte B-cytochrome
Documentation
References
[1]. Cipriano A, et al. NADPH Oxidases: From Molecular Mechanisms to Current Inhibitors. J Med Chem. 2023 Sep 14;66(17):11632-11655. [Content Brief]
[2]. Paclet MH, et al. Regulation of Neutrophil NADPH Oxidase, NOX2: A Crucial Effector in Neutrophil Phenotype and Function. Front Cell Dev Biol. 2022 Jul 14;10:945749. [Content Brief]
[3]. Altenhöfer S, et al. Evolution of NADPH Oxidase Inhibitors: Selectivity and Mechanisms for Target Engagement. Antioxid Redox Signal. 2015 Aug 10;23(5):406-27. [Content Brief]
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