NOX2 Antibody (YA6330)

Cat. No.: HY-P86638: Data Sheet User Guide for Antibodies
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NOX2 Antibody (YA6330) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NOX2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

NOX2 Antibody (YA6330) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NOX2.

Host

Rabbit

Clonality

Monoclonal

Molecular Weight

Predicted band size: 65 kDa;

Observed band size: 65 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P04839

Gene ID

1536 [NCBI]

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-5000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-20000
IP IP: Immunoprecipitation	1:50-200

Purity	Protein A	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Catalytic subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (PubMed:15338276, PubMed:36241643, PubMed:36413210, PubMed:38355798). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction (Probable) (PubMed:38355798). Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane (PubMed:19028840, PubMed:38355798). This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox (By similarity). NADPH oxidase complex assembly is impaired through interaction with NRROS (By similarity)

Subcellular Localization：Cell membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Protein levels were detected in neutrophils.

Subunit：Component of the phagocyte NADPH oxidase core complex/cytochrome b558 complex, composed of CYBB (heavy chain (beta)) and CYBA (light chain (alpha)) (PubMed:3600769, PubMed:36241643, PubMed:36413210). Component of the phagocyte NADPH oxidase complex composed of an obligatory core heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic regulatory subunits NCF1/p47-phox, NCF2/p67-phox, NCF4/p40-phox and the small GTPase RAC1 or RAC2 (PubMed:15338276, PubMed:19028840, PubMed:38355798). Interacts with NCF1 (phosphorylated form) (PubMed:19028840, PubMed:9224653). Interacts with NCF2; the interaction is enhanced in the presence of GBP7 (PubMed:19028840, PubMed:38355798). Interacts with RAC2 (PubMed:19028840). Interacts with RAC1 (PubMed:38355798). Interacts with calprotectin (S100A8/9) (PubMed:22808130). Interacts with NRROS; the interaction is direct and impairs formation of a stable NADPH oxidase complex (By similarity). Interacts with CYBC1; CYBC1 may act as a chaperone stabilizing Cytochrome b-245 heterodimer (PubMed:28351984). The CYBA-CYBB complex interacts with GBP7 (By similarity)

Synonyms

CYBB; NOX2; Cytochrome b-245 heavy chain; CGD91-phox; Cytochrome b; 558; subunit beta; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2Neutrophil cytochrome b 91 kDa polypeptide; Superoxide-generating NADPH oxidase heavy chain subunit; gp91-1; gp91-phox; p22 phagocyte B-cytochrome

Documentation

User Guide for Antibodies (1077 KB)