ATP2C1 Antibody (YA4144)

Cat. No.: HY-P84447: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

ATP2C1 Antibody (YA4144) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ATP2C1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

ATP2C1 Antibody (YA4144) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ATP2C1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 101 kDa;

Observed band size: 101 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Monkey

SwissProt ID

P98194

Gene ID

27032 [NCBI]

Immunogen

Purified recombinant fragment of ATP2C1 aa 119-269.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from PC-3 (lane 2(20μg), HT-29 (lane 3(20μg), SiHa (lane 4(20μg) and Raji (lane 5(20μg) using ATP2C1 Antibody (HY-P84447) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from A431(lane 1(40μg) ), Hela(lane 2(40μg) ), HEK293(lane 3(40μg) ) and K562(lane 4(40μg) )using ATP2C1 antibody(30001P). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，20035, 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：ATP-driven pump that supplies the Golgi apparatus with Ca(2+) and Mn(2+) ions, both essential cofactors for processing and trafficking of newly synthesized proteins in the secretory pathway (PubMed:12707275, PubMed:16192278, PubMed:20439740, PubMed:21187401, PubMed:30923126). Within a catalytic cycle, acquires Ca(2+) or Mn(2+) ions on the cytoplasmic side of the membrane and delivers them to the lumenal side. The transfer of ions across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing to outward-facing state (PubMed:16192278, PubMed:16332677, PubMed:30923126). Plays a primary role in the maintenance of Ca(2+) homeostasis in the trans-Golgi compartment with a functional impact on Golgi and post-Golgi protein sorting as well as a structural impact on cisternae morphology (PubMed:14632183, PubMed:20439740). Responsible for loading the Golgi stores with Ca(2+) ions in keratinocytes, contributing to keratinocyte differentiation and epidermis integrity (PubMed:10615129, PubMed:14632183, PubMed:20439740). Participates in Ca(2+) and Mn(2+) ions uptake into the Golgi store of hippocampal neurons and regulates protein trafficking required for neural polarity (By similarity). May also play a role in the maintenance of Ca(2+) and Mn(2+) homeostasis and signaling in the cytosol while preventing cytotoxicity (PubMed:21187401)

Subcellular Localization：Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein; Golgi apparatus, Golgi stack membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:It has been found in most tissues except the colon, thymus, spleen, and leukocytes (PubMed:15831496) . It is expressed in keratinocytes (protein level) (PubMed:14632183, PubMed:15831496) .

Isoforms & Post-Translational Modification：P98194 has 9 isomers: P98194-1: 100577 Da (predicted); P98194-2: 96960 Da (predicted); P98194-3: 101163 Da (predicted); P98194-4: 98733 Da (predicted); P98194-5: 103007 Da (predicted); P98194-6: 105057 Da (predicted); P98194-7: 107143 Da (predicted); P98194-8: 103524 Da (predicted); P98194-9: 104032 Da (predicted).

Subunit：Monomer. Homodimer

RRID

AB_3718908

Synonyms

HHD; BCPM; PMR1; SPCA1

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)