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ATP2C1 Antibody (YA4144)

(Synonyms: HHD; BCPM; PMR1; SPCA1)
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Based on 1 Customer Validation

ATP2C1 Antibody (YA4144) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ATP2C1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ELISA

  • Reactivity :

    Human, Monkey

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ATP2C1 Antibody (YA4144)
  • Experimental Validation Results for ATP2C1 Antibody (YA4144)
  • ATP2C1 Antibody (YA4144) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:10000

Product Details

Description

ATP2C1 Antibody (YA4144) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ATP2C1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Monkey
  • Observed Molecular Weight
    Observed band size: 101 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 101 kDa
Immunogen

Purified recombinant fragment of ATP2C1 aa 119-269.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718908

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ATP2C1 Antibody (YA4144)
    Western blot analysis of extracts from PC-3 (lane 2(20μg), HT-29 (lane 3(20μg), SiHa (lane 4(20μg) and Raji (lane 5(20μg) using ATP2C1 Antibody (HY-P84447) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ATP2C1 Antibody (YA4144)
    Western blot analysis of extracts from A431(lane 1(40μg) )

Background

  • Function

    ATP2C1 is an ATP-driven pump that supplies the Golgi apparatus with Ca(2+) and Mn(2+) ions, both essential cofactors for processing and trafficking of newly synthesized proteins in the secretory pathway. Within a catalytic cycle, acquires Ca(2+) or Mn(2+) ions on the cytoplasmic side of the membrane and delivers them to the lumenal side. The transfer of ions across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing to outward-facing state. Plays a primary role in the maintenance of Ca(2+) homeostasis in the trans-Golgi compartment with a functional impact on Golgi and post-Golgi protein sorting as well as a structural impact on cisternae morphology. Responsible for loading the Golgi stores with Ca(2+) ions in keratinocytes, contributing to keratinocyte differentiation and epidermis integrity. Participates in Ca(2+) and Mn(2+) ions uptake into the Golgi store of hippocampal neurons and regulates protein trafficking required for neural polarity. May also play a role in the maintenance of Ca(2+) and Mn(2+) homeostasis and signaling in the cytosol while preventing cytotoxicity[1][2][3][4][5][6][7][8].

  • Subcellular Localization

    Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein; Golgi apparatus, Golgi stack membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:It has been found in most tissues except the colon, thymus, spleen, and leukocytes (PubMed:15831496) . It is expressed in keratinocytes (protein level) (PubMed:14632183, PubMed:15831496) .

  • Isoforms & Post-Translational Modification

    P98194 has 9 isomers: P98194-1: 100577 Da (predicted); P98194-2: 96960 Da (predicted); P98194-3: 101163 Da (predicted); P98194-4: 98733 Da (predicted); P98194-5: 103007 Da (predicted); P98194-6: 105057 Da (predicted); P98194-7: 107143 Da (predicted); P98194-8: 103524 Da (predicted); P98194-9: 104032 Da (predicted).

  • Subunit

    Monomer. Homodimer

  • SwissProt ID

    P98194

  • Gene ID
    27032 [NCBI]
  • Synonyms

    HHD; BCPM; PMR1; SPCA1

ATP2C1 Antibody (YA4144) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from PC-3 (lane 2(20μg), HT-29 (lane 3(20μg), SiHa (lane 4(20μg) and Raji (lane 5(20μg) using ATP2C1 Antibody (HY-P84447) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A431(lane 1(40μg) )

  • ATP2C1 Antibody (YA4144) Product Image
    1/16
  • Experimental Validation Results for ATP2C1 Antibody (YA4144)
  • Experimental Validation Results for ATP2C1 Antibody (YA4144)
  • ATP2C1 Antibody (YA4144) Product Image
  • Experimental Validation Results for ATP2C1 Antibody (YA4144)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from PC-3 (lane 2(20μg), HT-29 (lane 3(20μg), SiHa (lane 4(20μg) and Raji (lane 5(20μg) using ATP2C1 Antibody (HY-P84447) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ATP2C1 Antibody (YA4144)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A431(lane 1(40μg) )
  • Experimental Validation Results for ATP2C1 Antibody (YA4144)
  • Experimental Validation Results for ATP2C1 Antibody (YA4144)
  • ATP2C1 Antibody (YA4144) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from PC-3 (lane 2(20μg), HT-29 (lane 3(20μg), SiHa (lane 4(20μg) and Raji (lane 5(20μg) using ATP2C1 Antibody (HY-P84447) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ATP2C1 Antibody (YA4144) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A431(lane 1(40μg) )

  • ATP2C1 Antibody (YA4144) Product Image WB
  • ATP2C1 Antibody (YA4144) Product Image WB