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DRD2 Antibody

(Synonyms: DRD2_HUMAN; D(2) dopamine receptor; Dopamine D2 receptor; dopamine receptor D2; D2R; D2DR; )
Customer Review

Based on 1 Customer Validation

DRD2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to DRD2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Mouse, Rat

  • Formulation:

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for DRD2 Antibody
  • DRD2 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-2000

Product Details

Description

DRD2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to DRD2.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Mouse, Rat Predicted Reactivity: Human
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 51 kDa
Species Reactivity Database
Immunogen

KLH conjugated synthetic peptide derived from human DRD2: 201-300/443

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103203

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for DRD2 Antibody
    Western blot analysis of extracts from SH-SY5Y (lane 2(20μg), U-87 (lane 3(20μg),THP-1 (lane 4(20μg) using DRD2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase (PubMed:21645528). Positively regulates postnatal regression of retinal hyaloid vessels via suppression of VEGFR2/KDR activity, downstream of OPN5 (By similarity)

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein; Golgi apparatus membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Expressed in the anterior pituitary gland; Expressed in the anterior pituitary gland

  • Isoforms & Post-Translational Modification

    P14416 has 3 isomers: P14416-1: 50619 Da (predicted); P14416-2: 47347 Da (predicted); P14416-3: 50847 Da (predicted).
    Palmitoylated. Palmitoylation which is required for proper localization to the plasma membrane and stability of the receptor could be carried on by ZDHHC4, ZDHHC3 and ZDHHC8

  • Subunit

    Forms homo- and heterooligomers with DRD4 (PubMed:21184734). The interaction with DRD4 may modulate agonist-induced downstream signaling (PubMed:21184734). Interacts with CADPS and CADPS2 (PubMed:15857609). Interacts with GPRASP1, PPP1R9B and CLIC6 (By similarity). Interacts with ARRB2 (By similarity). Interacts with HTR2A (PubMed:21645528). Interacts with GNAI2 isoform sGi2, the interaction allows the creation of an intracellular pool of DRD2 that can be released to cell surface upon agonist stimulation (PubMed:17550964). Interacts with DRD1 (By similarity). Interacts with KCNA2 (By similarity)

  • SwissProt ID

    P14416

  • Gene ID
    1813 [NCBI]
  • Synonyms

    DRD2_HUMAN; D(2) dopamine receptor; Dopamine D2 receptor; dopamine receptor D2; D2R; D2DR;

DRD2 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from SH-SY5Y (lane 2(20μg), U-87 (lane 3(20μg),THP-1 (lane 4(20μg) using DRD2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • DRD2 Antibody Product Image
    1/16
  • Experimental Validation Results for DRD2 Antibody
  • DRD2 Antibody Product Image
  • Experimental Validation Results for DRD2 Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from SH-SY5Y (lane 2(20μg), U-87 (lane 3(20μg),THP-1 (lane 4(20μg) using DRD2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for DRD2 Antibody
  • DRD2 Antibody Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from SH-SY5Y (lane 2(20μg), U-87 (lane 3(20μg),THP-1 (lane 4(20μg) using DRD2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • DRD2 Antibody Product Image WB