Caspase-10 Antibody (YA2366)(PBS only)

Cat. No.: HY-P82621A: Data Sheet User Guide for Antibodies
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Caspase-10 Antibody (YA2366) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Caspase-10.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

Caspase-10 Antibody (YA2366) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Caspase-10.

Host

Rabbit

Molecular Weight

Predicted band size: 59 kDa;

Observed band size: 59 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q92851

Gene ID

843 [NCBI]

Immunogen

Recombinant protein of human Caspase-10

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP8 and CASP9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC; Isoform 7 can enhance NF-kappaB activity but promotes only slight apoptosis; Isoform C is proteolytically inactive

Expression：
Tissue_specificity:It can be detected in most tissues. The lowest expression levels are found in the brain, kidneys, prostate, testes, and colon.

Isoforms & Post-Translational Modification：Q92851 has 7 isomers: Q92851-1: 58951 Da (predicted); Q92851-2: 54566 Da (predicted); Q92851-4: 58994 Da (predicted); Q92851-3: 31419 Da (predicted); Q92851-5: 54523 Da (predicted); Q92851-6: 51785 Da (predicted); Q92851-7: 28364 Da (predicted).
Cleavage by granzyme B and autocatalytic activity generate the two active subunits

Subunit：Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 23/17 kDa (p23/17) (depending on the splicing events) and a 12 kDa (p12) subunit (By similarity). Self-associates. Interacts with FADD and CASP8. Found in a Fas signaling complex consisting of FAS, FADD, CASP8 and CASP10. Interacts with RFFL and RNF34; negatively regulate CASP10 through proteasomal degradation. Interacts with RIOK3

Research Field

Cell Biology

Synonyms

CASP10; MCH4; Caspase-10; CASP-10; Apoptotic protease Mch-4; FAS-associated death domain protein interleukin-1B-converting enzyme 2; FLICE2; ICE-like apoptotic protease 4

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/ht-29-cell.html [Content Brief]