FYB Antibody (YA973)
(Synonyms: ADAP; Fyb; FYN binding protein; p120/p130; SLAP130)FYB Antibody (YA973) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FYB.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
FYB Antibody (YA973) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FYB.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 120 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 85 kDa
Purified recombinant human FYB protein fragments expressed in E.coli.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
FYB acts as an adapter protein of the FYN and LCP2 signaling cascades in T-cells. May play a role in linking T-cell signaling to remodeling of the actin cytoskeleton. Modulates the expression of IL2. Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells[1][2][3].
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Subcellular Localization
Cytoplasm; Nucleus; Cell junction
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Expression
Tissue_specificity:It is expressed in hematopoietic tissues (such as myeloid cells and T cells) , spleen, and thymus. It is not expressed in B cells and non-lymphoid tissues. -
Isoforms & Post-Translational Modification
O15117 has 3 isomers: O15117-1: 85387 Da (predicted); O15117-2: 90606 Da (predicted); O15117-3: 91651 Da (predicted).
T-cell receptor ligation leads to increased tyrosine phosphorylation -
Subunit
Part of a complex consisting of SKAP2, FYB1, LILRB3 and PTPNS1 (By similarity).
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SwissProt ID
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Synonyms
ADAP; Fyb; FYN binding protein; p120/p130; SLAP130
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Research Field
Signal Transduction
Documentation
References
[1]. Krause M, et al. Fyn-binding protein (Fyb)/SLP-76-associated protein (SLAP), Ena/vasodilator-stimulated phosphoprotein (VASP) proteins and the Arp2/3 complex link T cell receptor (TCR) signaling to the actin cytoskeleton. J Cell Biol. 2000 Apr 3;149(1):181-94. [Content Brief]
[2]. Kliche S, et al. The ADAP/SKAP55 signaling module regulates T-cell receptor-mediated integrin activation through plasma membrane targeting of Rap1. Mol Cell Biol. 2006 Oct;26(19):7130-44. [Content Brief]
[3]. Huang Y, et al. Deficiency of ADAP/Fyb/SLAP-130 destabilizes SKAP55 in Jurkat T cells. J Biol Chem. 2005 Jun 24;280(25):23576-83. [Content Brief]
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