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GCLC Antibody (YA2898)(PBS only)

(Synonyms: GCLC; GLCL; GLCLC; Glutamate--cysteine ligase catalytic subunit; GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase)

GCLC Antibody (YA2898) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GCLC.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for GCLC Antibody (YA2898)(PBS only)
  • GCLC Antibody (YA2898)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

GCLC Antibody (YA2898) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GCLC.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 73 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 73 kDa
Immunogen

Recombinant protein of human GCLC

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GCLC Antibody (YA2898)(PBS only)
    Western blot analysis of extracts from MCF7 (lane 2(20μg)),A549 (lane 3(20μg)),C6 (lane 4(20μg)) ,Raw264.7 (lane 5(20μg)) and Jurkat (lane 6(20μg)) using GCLC Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    GCLC catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine and participates in the first and rate-limiting step in glutathione biosynthesis

  • Subunit

    Heterodimer of a catalytic heavy chain and a regulatory light chain

  • SwissProt ID

    P48506

  • Gene ID
    2729 [NCBI]
  • Synonyms

    GCLC; GLCL; GLCLC; Glutamate--cysteine ligase catalytic subunit; GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase

  • Research Field

    Signal Transduction

GCLC Antibody (YA2898)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF7 (lane 2(20μg)),A549 (lane 3(20μg)),C6 (lane 4(20μg)) ,Raw264.7 (lane 5(20μg)) and Jurkat (lane 6(20μg)) using GCLC Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • GCLC Antibody (YA2898)(PBS only) Product Image
    1/16
  • Experimental Validation Results for GCLC Antibody (YA2898)(PBS only)
  • GCLC Antibody (YA2898)(PBS only) Product Image
  • Experimental Validation Results for GCLC Antibody (YA2898)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF7 (lane 2(20μg)),A549 (lane 3(20μg)),C6 (lane 4(20μg)) ,Raw264.7 (lane 5(20μg)) and Jurkat (lane 6(20μg)) using GCLC Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GCLC Antibody (YA2898)(PBS only)
  • GCLC Antibody (YA2898)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF7 (lane 2(20μg)),A549 (lane 3(20μg)),C6 (lane 4(20μg)) ,Raw264.7 (lane 5(20μg)) and Jurkat (lane 6(20μg)) using GCLC Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • GCLC Antibody (YA2898)(PBS only) Product Image WB