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WDR77 Antibody (YA655)

(Synonyms: WD repeat domain 77; p44; MEP50; MEP-50; HKMT1069; Nbla10071; p44/Mep50)
Customer Review

Based on 1 Customer Validation

WDR77 Antibody (YA655) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to WDR77.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for WDR77 Antibody (YA655)
  • Experimental Validation Results for WDR77 Antibody (YA655)
  • Experimental Validation Results for WDR77 Antibody (YA655)
  • WDR77 Antibody (YA655) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

WDR77 Antibody (YA655) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to WDR77.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 37 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human WDR77.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102760

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for WDR77 Antibody (YA655)
    Western blot analysis of extracts from C6(lane 2(20ug) , K562(lane 3(20ug) and NIH/3T3(lane 4(20ug) using WDR77 Antibody (HY-P80931) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for WDR77 Antibody (YA655)
    Immunocytochemistry analysis of Hela cells labeling WDR77 with WDR77 Antibody (HY-P80931) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with WDR77 Antibody (HY-P80931) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for WDR77 Antibody (YA655)
    Immunocytochemistry analysis of Hela cells labeling WDR77 with WDR77 Antibody (HY-P80931) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with WDR77 Antibody (HY-P80931) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones (PubMed:11756452). This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage (PubMed:23071334)

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Expression


    Tissue_specificity:It is highly expressed in the heart, skeletal muscle, spleen, testes, uterus, prostate, and thymus. In the testes, it is expressed in germ cells and interstitial cells, but not in peritubular muscle cells and Sertoli cells. It is expressed in prostate cancer, seminoma, and interstitial cell tumors.

  • Isoforms & Post-Translational Modification

    Q9BQA1 has 2 isomers: Q9BQA1-1: 36724 Da (predicted); Q9BQA1-2: 29651 Da (predicted).

  • Subunit

    Component of the methylosome complex composed of PRMT5, WDR77 and CLNS1A (PubMed:18984161, PubMed:21081503). Found in a complex composed of PRMT5, WDR77 and RIOK1 (PubMed:21081503). RIOK1 and CLNS1A bound directly to PRMT5 at the same binding site, in a mutually exclusive manner, which allows the recruitment of distinct methylation substrates, such as nucleolin/NCL and Sm proteins, respectively (PubMed:21081503). Found in a complex with the component of the methylosome, PRMT5, CLNS1A, WDR77, PRMT1 and ERH (PubMed:25284789). Directly interacts with PRMT5, as well as with several Sm proteins, including SNRPB and SNRPD2 and, more weakly, SNRPD3 and SNRPE (PubMed:11756452, PubMed:33376131). Forms a compact hetero-octamer with PRMT5, decorating the outer surface of a PRMT5 tetramer. Interacts with SUZ12 and histone H2A/H2AC20, but not with histones H2B, H3 nor H4 (PubMed:16712789). Interacts with CTDP1 and LSM11 (PubMed:12560496, PubMed:16087681). Interacts with APEX1, AR and NKX3-1 (PubMed:12972618, PubMed:19188445). Interacts with CHTOP (PubMed:25284789). Interacts with FAM47E. Interacts with TSC22D2 (PubMed:27337956)

  • SwissProt ID

    Q9BQA1

  • Gene ID
    79084 [NCBI]
  • Synonyms

    WD repeat domain 77; p44; MEP50; MEP-50; HKMT1069; Nbla10071; p44/Mep50

  • Research Field

    Signal Transduction

WDR77 Antibody (YA655) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from C6(lane 2(20ug) , K562(lane 3(20ug) and NIH/3T3(lane 4(20ug) using WDR77 Antibody (HY-P80931) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling WDR77 with WDR77 Antibody (HY-P80931) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with WDR77 Antibody (HY-P80931) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling WDR77 with WDR77 Antibody (HY-P80931) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with WDR77 Antibody (HY-P80931) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • WDR77 Antibody (YA655) Product Image
    1/16
  • Experimental Validation Results for WDR77 Antibody (YA655)
  • Experimental Validation Results for WDR77 Antibody (YA655)
  • Experimental Validation Results for WDR77 Antibody (YA655)
  • WDR77 Antibody (YA655) Product Image
  • Experimental Validation Results for WDR77 Antibody (YA655)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from C6(lane 2(20ug) , K562(lane 3(20ug) and NIH/3T3(lane 4(20ug) using WDR77 Antibody (HY-P80931) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for WDR77 Antibody (YA655)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling WDR77 with WDR77 Antibody (HY-P80931) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with WDR77 Antibody (HY-P80931) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for WDR77 Antibody (YA655)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling WDR77 with WDR77 Antibody (HY-P80931) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with WDR77 Antibody (HY-P80931) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for WDR77 Antibody (YA655)
  • Experimental Validation Results for WDR77 Antibody (YA655)
  • Experimental Validation Results for WDR77 Antibody (YA655)
  • WDR77 Antibody (YA655) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from C6(lane 2(20ug) , K562(lane 3(20ug) and NIH/3T3(lane 4(20ug) using WDR77 Antibody (HY-P80931) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • WDR77 Antibody (YA655) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling WDR77 with WDR77 Antibody (HY-P80931) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with WDR77 Antibody (HY-P80931) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • WDR77 Antibody (YA655) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling WDR77 with WDR77 Antibody (HY-P80931) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with WDR77 Antibody (HY-P80931) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • WDR77 Antibody (YA655) Product Image WB
  • WDR77 Antibody (YA655) Product Image ICC
  • WDR77 Antibody (YA655) Product Image ICC