Pyruvate Dehydrogenase E1 alpha Antibody (YA3708)

Cat. No.: HY-P84011: Data Sheet User Guide for Antibodies
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Pyruvate Dehydrogenase E1 alpha Antibody (YA3708) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Pyruvate Dehydrogenase E1 alpha.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Pyruvate Dehydrogenase E1 alpha Antibody (YA3708) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Pyruvate Dehydrogenase E1 alpha.

Host

Mouse

Clonality

Monoclonal Antibody

Molecular Weight

Predicted band size: 43 kDa;

Observed band size: 43 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P08559

Gene ID

5160 [NCBI]

Immunogen

Purified recombinant fragment of human PDHA1(AA: 241-390) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IP IP: Immunoprecipitation	1:200-1:300
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle

Subcellular Localization：Mitochondrion matrix

Expression：
Tissue_specificity:general expression

Isoforms & Post-Translational Modification：P08559 has 4 isomers: P08559-1: 43296 Da (predicted); P08559-2: 44109 Da (predicted); P08559-3: 40188 Da (predicted); P08559-4: 47580 Da (predicted).
Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme; for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation;Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3 (By similarity)

Subunit：Heterotetramer of two PDHA1 and two PDHB subunits. The heterotetramer interacts with DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules

Synonyms

PDHA; PDHAD; PHE1A; PDHCE1A

Documentation

User Guide for Antibodies (1077 KB)