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BCR Antibody (YA2746)(PBS only)

(Synonyms: ALL; CML; PHL; BCR1; D22S11; D22S662)

BCR Antibody (YA2746) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BCR.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for BCR Antibody (YA2746)(PBS only)
  • BCR Antibody (YA2746)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:20

Product Details

Description

BCR Antibody (YA2746) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BCR.

  • Host Rabbit
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 160 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 143 kDa
Immunogen

A synthetic peptide of human Bcr aa1254-1271.

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for BCR Antibody (YA2746)(PBS only)
    Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), and HeLa (lane 2, 20 μg) using Bcr Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    BCR is a Protein with a unique structure having two opposing regulatory activities toward small GTP-binding proteins. The C-terminus is a GTPase-activating protein (GAP) domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. Accelerates the intrinsic rate of GTP hydrolysis of RAC1 or CDC42, leading to down-regulation of the active GTP-bound form. The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form. The amino terminus contains an intrinsic kinase activity. Functions as an important negative regulator of neuronal RAC1 activity. Regulates macrophage functions such as CSF1-directed motility and phagocytosis through the modulation of RAC1 activity. Plays a major role as a RHOA GEF in keratinocytes being involved in focal adhesion formation and keratinocyte differentiation[1][2][3][4][5].

  • Subcellular Localization

    Postsynaptic density; Cell projection, dendritic spine; Cell projection, axon; Synapse

  • Isoforms & Post-Translational Modification

    P11274 has 2 isomers: P11274-1: 142819 Da (predicted); P11274-2: 137729 Da (predicted).
    Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2

  • Subunit

    Homotetramer. Interacts with PDZK1 (PubMed:15494376). May interact with CCPG1 (By similarity). Interacts with FES/FPS, ABL1, PIK3R1 and GRB2 (PubMed:15302586, PubMed:1712671, PubMed:9407116). Interacts with HCK (PubMed:9407116). Interacts with SH2D5 (PubMed:25331951). Interacts with DLG4 (PubMed:20962234)

  • SwissProt ID

    P11274

  • Gene ID
    613 [NCBI]
  • Synonyms

    ALL; CML; PHL; BCR1; D22S11; D22S662

  • Research Field

    Signal Transduction

BCR Antibody (YA2746)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), and HeLa (lane 2, 20 μg) using Bcr Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • BCR Antibody (YA2746)(PBS only) Product Image
    1/16
  • Experimental Validation Results for BCR Antibody (YA2746)(PBS only)
  • BCR Antibody (YA2746)(PBS only) Product Image
  • Experimental Validation Results for BCR Antibody (YA2746)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), and HeLa (lane 2, 20 μg) using Bcr Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for BCR Antibody (YA2746)(PBS only)
  • BCR Antibody (YA2746)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), and HeLa (lane 2, 20 μg) using Bcr Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • BCR Antibody (YA2746)(PBS only) Product Image WB