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VDAC1 Antibody (YA016)

(Synonyms: OMP2, VDAC1, YNL055C, N2441, YNL2441C, POR1, Non-selective voltage-gated ion channel 1, Outer mitochondrial membrane protein porin 1, Voltage-dependent anion-selective channel protein 1, VDAC-1)
1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

VDAC1 Antibody is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to VDAC1. It can be used as a loading control antibody.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC, IF-Tissue

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC IHC-P
  • Experimental Validation Results for VDAC1 Antibody (YA016)
  • Experimental Validation Results for VDAC1 Antibody (YA016)
  • Experimental Validation Results for VDAC1 Antibody (YA016)
  • Experimental Validation Results for VDAC1 Antibody (YA016)
  • Experimental Validation Results for VDAC1 Antibody (YA016)
  • Experimental Validation Results for VDAC1 Antibody (YA016)
  • VDAC1 Antibody (YA016) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
Dilution Ratio 1:10000 1:50-100 1:1000 1:500-1:1,000 1:200

Product Details

Description

VDAC1 Antibody is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to VDAC1. It can be used as a loading control antibody.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 31 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 31 kDa
Species Reactivity Database
Immunogen

Synthetic peptide within N-terminal human VDAC1.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102459

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for VDAC1 Antibody (YA016)
    Western blot analysis of extracts from MDA-MB231(lane 2(20μg), Hela(lane 3(20μg) and K562(lane 4(20μg) using VDAC1(HY-P80369) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for VDAC1 Antibody (YA016)
    Western blot analysis of extracts from MDA-MB-231 (lane 2(40μg), HeLa (lane 3(40μg), K-562 (lane 4(40μg)using VDAC1 Antibody (YA016). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for VDAC1 Antibody (YA016)
    Immunocytochemistry analysis of Hela cells labeling VDAC1 with VDAC1 Antibody (HY-P80369) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with VDAC1 Antibody (HY-P80369) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for VDAC1 Antibody (YA016)
    Immunocytochemistry analysis of HepG2 cells labeling VDAC1with VDAC1 Antibody (HY-P80369) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with VDAC1 Antibody (HY-P80369)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for VDAC1 Antibody (YA016)
    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using VDAC1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80369, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for VDAC1 Antibody (YA016)
    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using VDAC1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80369, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    VDAC1 (Voltage-Dependent Anion Channel 1) is the major pore-forming protein of the mitochondrial outer membrane and mediates the exchange of metabolites and ions between mitochondria and the cytosol, thereby linking mitochondrial activity to cellular energy homeostasis and survival programs[1]. Mechanistically, VDAC1 regulates metabolic flux, ATP transport, calcium signaling, and mitochondrial function, placing it at the intersection of bioenergetic control and apoptosis-related pathways[1][2]. In disease-associated contexts, VDAC1 has been extensively studied in cancer, neurodegeneration, and other disorders characterized by mitochondrial dysfunction, where altered channel activity, protein interactions, or expression levels influence cell fate decisions and stress responses[2][3]. VDAC1 also participates in apoptosis-related mechanisms through interactions with Bcl-2 family proteins and through structural transitions associated with oligomerization, processes that can affect mitochondrial membrane permeabilization and downstream death signaling[2][4]. Compared with related isoforms, VDAC1 shares substantial structural homology with VDAC2 and VDAC3 but is generally recognized as the predominant isoform involved in mitochondrial metabolite transport and apoptosis-associated signaling, making it the most frequently investigated family member in mechanistic studies[3]. For experimental applications, VDAC1 is widely used as a mitochondrial biology and cell-death research target, and modulation of VDAC1 channel activity or oligomerization has been explored as a strategy to investigate mitochondrial dysfunction, apoptosis, and disease-relevant signaling networks[2][4].

  • Subcellular Localization

    Mitochondrion outer membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein; Membrane raft; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Expression in erythrocytes (protein level) (PubMed: 27641616) . Expression in heart, liver, and skeletal muscle (PubMed: 8420959) .

  • Subunit

    Homodimer and homotrimer; in response to cyclic AMP or calcium; oligomerization is required for scramblase activity (PubMed:30061676, PubMed:38065946).

  • SwissProt ID

    P21796

  • Gene ID
    7416 [NCBI]
  • Synonyms

    OMP2, VDAC1, YNL055C, N2441, YNL2441C, POR1, Non-selective voltage-gated ion channel 1, Outer mitochondrial membrane protein porin 1, Voltage-dependent anion-selective channel protein 1, VDAC-1

  • Research Field

    Tags & Cell Markers

[1]. VDAC1 gene information from NCBI.

[2]. Shoshan-Barmatz V, et al. The mitochondrial voltage-dependent anion channel 1 in tumor cells. Biochim Biophys Acta. 2015 Oct;1848(10 Pt B):2547-75. [Content Brief]

[3]. De Pinto V, et al. Characterization of human VDAC isoforms: a peculiar function for VDAC3? Biochim Biophys Acta. 2010 Jun-Jul;1797(6-7):1268-75. [Content Brief]

[4]. Daniilidis M, et al. Structural basis of apoptosis induction by the mitochondrial voltage-dependent anion channel. Nat Commun. 2025 Oct 27;16(1):9481. [Content Brief]

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VDAC1 Antibody (YA016) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from MDA-MB231(lane 2(20μg), Hela(lane 3(20μg) and K562(lane 4(20μg) using VDAC1(HY-P80369) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from MDA-MB-231 (lane 2(40μg), HeLa (lane 3(40μg), K-562 (lane 4(40μg)using VDAC1 Antibody (YA016). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling VDAC1 with VDAC1 Antibody (HY-P80369) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with VDAC1 Antibody (HY-P80369) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling VDAC1with VDAC1 Antibody (HY-P80369) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with VDAC1 Antibody (HY-P80369)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using VDAC1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80369, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using VDAC1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80369, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • VDAC1 Antibody (YA016) Product Image
    1/16
  • Experimental Validation Results for VDAC1 Antibody (YA016)
  • Experimental Validation Results for VDAC1 Antibody (YA016)
  • Experimental Validation Results for VDAC1 Antibody (YA016)
  • Experimental Validation Results for VDAC1 Antibody (YA016)
  • Experimental Validation Results for VDAC1 Antibody (YA016)
  • Experimental Validation Results for VDAC1 Antibody (YA016)
  • VDAC1 Antibody (YA016) Product Image
  • Experimental Validation Results for VDAC1 Antibody (YA016)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from MDA-MB231(lane 2(20μg), Hela(lane 3(20μg) and K562(lane 4(20μg) using VDAC1(HY-P80369) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for VDAC1 Antibody (YA016)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from MDA-MB-231 (lane 2(40μg), HeLa (lane 3(40μg), K-562 (lane 4(40μg)using VDAC1 Antibody (YA016). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for VDAC1 Antibody (YA016)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling VDAC1 with VDAC1 Antibody (HY-P80369) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with VDAC1 Antibody (HY-P80369) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for VDAC1 Antibody (YA016)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling VDAC1with VDAC1 Antibody (HY-P80369) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with VDAC1 Antibody (HY-P80369)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for VDAC1 Antibody (YA016)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using VDAC1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80369, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for VDAC1 Antibody (YA016)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using VDAC1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80369, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for VDAC1 Antibody (YA016)
  • Experimental Validation Results for VDAC1 Antibody (YA016)
  • Experimental Validation Results for VDAC1 Antibody (YA016)
  • Experimental Validation Results for VDAC1 Antibody (YA016)
  • Experimental Validation Results for VDAC1 Antibody (YA016)
  • Experimental Validation Results for VDAC1 Antibody (YA016)
  • VDAC1 Antibody (YA016) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from MDA-MB231(lane 2(20μg), Hela(lane 3(20μg) and K562(lane 4(20μg) using VDAC1(HY-P80369) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • VDAC1 Antibody (YA016) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from MDA-MB-231 (lane 2(40μg), HeLa (lane 3(40μg), K-562 (lane 4(40μg)using VDAC1 Antibody (YA016). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • VDAC1 Antibody (YA016) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling VDAC1 with VDAC1 Antibody (HY-P80369) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with VDAC1 Antibody (HY-P80369) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • VDAC1 Antibody (YA016) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling VDAC1with VDAC1 Antibody (HY-P80369) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with VDAC1 Antibody (HY-P80369)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • VDAC1 Antibody (YA016) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using VDAC1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80369, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • VDAC1 Antibody (YA016) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using VDAC1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80369, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • VDAC1 Antibody (YA016) Product Image WB
  • VDAC1 Antibody (YA016) Product Image WB
  • VDAC1 Antibody (YA016) Product Image ICC
  • VDAC1 Antibody (YA016) Product Image ICC
  • VDAC1 Antibody (YA016) Product Image IHC-P
  • VDAC1 Antibody (YA016) Product Image IHC-P