IP3 Receptor Antibody (YA1596)

Cat. No.: HY-P81851: Data Sheet COA
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User Guide for Antibodies
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IP3 Receptor Antibody (YA1596) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IP3 Receptor.

For research use only. We do not sell to patients.

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10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
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IP3 Receptor Antibody (YA1596) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

IP3 Receptor Antibody (YA1596) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IP3 Receptor.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 314 kDa;

Observed band size: 314 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q14643

Gene ID

3708 [NCBI]

Immunogen

A synthesized peptide derived from human IP3 Receptor aa2200-2300.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Inositol 1,4,5-trisphosphate-gated calcium channel that, upon inositol 1,4,5-trisphosphate binding, mediates calcium release from the endoplasmic reticulum (ER) (PubMed:10620513, PubMed:27108797). Undergoes conformational changes upon ligand binding, suggesting structural flexibility that allows the channel to switch from a closed state, capable of interacting with its ligands such as 1,4,5-trisphosphate and calcium, to an open state, capable of transferring calcium ions across the ER membrane (By similarity). Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CAMK2 complex (By similarity). Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1 (By similarity). Part of a complex composed of HSPA9, ITPR1 and VDAC1 that regulates mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER lumen to the mitochondria intermembrane space thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix (By similarity). Regulates fertilization and egg activation by tuning the frequency and amplitude of calcium oscillations (By similarity)

Subcellular Localization：Endoplasmic reticulum membrane; Multi-pass membrane protein; Cytoplasmic vesicle, secretory vesicle membrane; Multi-pass membrane protein; Cytoplasm, perinuclear region

Expression：
Tissue_specificity:Widely expressed

Isoforms & Post-Translational Modification：Q14643 has 8 isomers: Q14643-1: 313929 Da (predicted); Q14643-2: 312163 Da (predicted); Q14643-3: 308539 Da (predicted); Q14643-4: 306773 Da (predicted); Q14643-5: 311135 Da (predicted); Q14643-6: 312003 Da (predicted); Q14643-7: 310237 Da (predicted); Q14643-8: 309209 Da (predicted).
Polyubiquitinated (By similarity). Polyubiquitination targets ITPR1 for proteasomal degradation (By similarity). Approximately 40% of the ITPR1-associated ubiquitin is monoubiquitin, and polyubiquitins are both 'Lys-48'- and 'Lys-63'-linked (By similarity);Phosphorylated by cAMP kinase (PKA) enhances calcium release (By similarity). Phosphorylation by PKA increases the interaction with inositol 1,4,5-trisphosphate and decreases the interaction with AHCYL1 (By similarity);Phosphorylated on tyrosine residues;Palmitoylated by ZDHHC6 in immune cells, leading to regulation of ITPR1 stability and function

Subunit：Homotetramer (By similarity). Homodimer (By similarity). Interacts with ERP44 in a pH-, redox state- and calcium-dependent manner which results in the inhibition the calcium channel activity (PubMed:15652484). The strength of this interaction inversely correlates with calcium concentration (PubMed:15652484). Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1 (By similarity). Interacts with IRAG1 (PubMed:16990611). Interacts with CABP1 (via N-terminus) (PubMed:12032348, PubMed:14685260). Interacts with TESPA1 (PubMed:23650607). Interacts (when not phosphorylated) with AHCYL1 (when phosphorylated); the interaction suppresses inositol 1,4,5-trisphosphate binding to ITPR1 and is increased in the presence of BCL2L10 (PubMed:16793548, PubMed:27995898). Interacts with AHCYL2 (with lower affinity than with AHCYL1) (PubMed:19220705). Interacts with BCL2L10; the interaction is increased in the presence of AHCLY1 (PubMed:27995898). Interacts with BOK (via BH4 domain); protects ITPR1 from proteolysis by CASP3 during apoptosis (PubMed:23884412). Interacts with TRPC4 (By similarity). Interacts with CHGA and CHGB (By similarity). Interacts with CALM1; this interaction inhibits inositol 1,4,5 trisphosphate binding in both the presence and absence of calcium and 1,4,5 trisphosphate-induced calcium release in the presence of calcium (By similarity). Interacts with the complex composed by ERLIN1, ERLIN2 and RNF170 through ERLIN2; this interaction triggers its ubiquitin-proteasomal degradation (By similarity). Interacts with HSPA9; this interaction couples ITPR1 to VDAC1 (By similarity)

RRID

AB_3103949

Database

Entrez Gene: 3708 Human ; 16438 Mouse ; 25262 Rat

SwissProt: Q14643 Human ; P11881 Mouse ; P29994 Rat

OMIM: 606658 Human

Research Field

Signal Transduction

Synonyms

5-trisphosphate receptor; Inositol 1; InsP3R1; IP3; IP3 receptor; IP3R 1; IP3R; IP3R1; Itpr1; SCA15; SCA16; SCA29

Documentation

Select Batch:

Data Sheet (232 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

IP3 Receptor Antibody (YA1596) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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IP3 Receptor Antibody (YA1596)

IP3 Receptor Antibody (YA1596) Featured Recommendations:

Product Detail

Background

Documentation

IP3 Receptor Antibody (YA1596) Related Classifications

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