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IP3 Receptor Antibody (YA1596)

(Synonyms: 5-trisphosphate receptor; Inositol 1; InsP3R1; IP3; IP3 receptor; IP3R 1; IP3R; IP3R1; Itpr1; SCA15; SCA16; SCA29)
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IP3 Receptor Antibody (YA1596) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IP3 Receptor.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • IP3 Receptor Antibody (YA1596) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

IP3 Receptor Antibody (YA1596) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IP3 Receptor.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 314 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 314 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human IP3 Receptor aa2200-2300.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103949

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    IP3 Receptor is an Inositol 1,4,5-trisphosphate-gated calcium channel that, upon inositol 1,4,5-trisphosphate binding, mediates calcium release from the endoplasmic reticulum (ER). Undergoes conformational changes upon ligand binding, suggesting structural flexibility that allows the channel to switch from a closed state, capable of interacting with its ligands such as 1,4,5-trisphosphate and calcium, to an open state, capable of transferring calcium ions across the ER membrane. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CAMK2 complex. Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1. Part of a complex composed of HSPA9, ITPR1 and VDAC1 that regulates mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER lumen to the mitochondria intermembrane space thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix. Regulates fertilization and egg activation by tuning the frequency and amplitude of calcium oscillations[1][2].

  • Subcellular Localization

    Endoplasmic reticulum membrane; Multi-pass membrane protein; Cytoplasmic vesicle, secretory vesicle membrane; Multi-pass membrane protein; Cytoplasm, perinuclear region

  • Expression


    Tissue_specificity:Widely expressed

  • Isoforms & Post-Translational Modification

    Q14643 has 8 isomers: Q14643-1: 313929 Da (predicted); Q14643-2: 312163 Da (predicted); Q14643-3: 308539 Da (predicted); Q14643-4: 306773 Da (predicted); Q14643-5: 311135 Da (predicted); Q14643-6: 312003 Da (predicted); Q14643-7: 310237 Da (predicted); Q14643-8: 309209 Da (predicted).
    Polyubiquitinated (By similarity). Polyubiquitination targets ITPR1 for proteasomal degradation (By similarity). Approximately 40% of the ITPR1-associated ubiquitin is monoubiquitin, and polyubiquitins are both 'Lys-48'- and 'Lys-63'-linked (By similarity);Phosphorylated by cAMP kinase (PKA) enhances calcium release (By similarity). Phosphorylation by PKA increases the interaction with inositol 1,4,5-trisphosphate and decreases the interaction with AHCYL1 (By similarity);Phosphorylated on tyrosine residues;Palmitoylated by ZDHHC6 in immune cells, leading to regulation of ITPR1 stability and function

  • Subunit

    Homotetramer (By similarity). Homodimer (By similarity). Interacts with ERP44 in a pH-, redox state- and calcium-dependent manner which results in the inhibition the calcium channel activity (PubMed:15652484). The strength of this interaction inversely correlates with calcium concentration (PubMed:15652484). Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1 (By similarity). Interacts with IRAG1 (PubMed:16990611). Interacts with CABP1 (via N-terminus) (PubMed:12032348, PubMed:14685260). Interacts with TESPA1 (PubMed:23650607). Interacts (when not phosphorylated) with AHCYL1 (when phosphorylated); the interaction suppresses inositol 1,4,5-trisphosphate binding to ITPR1 and is increased in the presence of BCL2L10 (PubMed:16793548, PubMed:27995898). Interacts with AHCYL2 (with lower affinity than with AHCYL1) (PubMed:19220705). Interacts with BCL2L10; the interaction is increased in the presence of AHCLY1 (PubMed:27995898). Interacts with BOK (via BH4 domain); protects ITPR1 from proteolysis by CASP3 during apoptosis (PubMed:23884412). Interacts with TRPC4 (By similarity). Interacts with CHGA and CHGB (By similarity). Interacts with CALM1; this interaction inhibits inositol 1,4,5 trisphosphate binding in both the presence and absence of calcium and 1,4,5 trisphosphate-induced calcium release in the presence of calcium (By similarity). Interacts with the complex composed by ERLIN1, ERLIN2 and RNF170 through ERLIN2; this interaction triggers its ubiquitin-proteasomal degradation (By similarity). Interacts with HSPA9; this interaction couples ITPR1 to VDAC1 (By similarity)

  • SwissProt ID

    Q14643

  • Gene ID
    3708 [NCBI]
  • Synonyms

    5-trisphosphate receptor; Inositol 1; InsP3R1; IP3; IP3 receptor; IP3R 1; IP3R; IP3R1; Itpr1; SCA15; SCA16; SCA29

  • Research Field

    Signal Transduction

[1]. Adkins CE, et al. Ca2+-calmodulin inhibits Ca2+ release mediated by type-1, -2 and -3 inositol trisphosphate receptors. Biochem J. 2000 Jan 15;345 Pt 2(Pt 2):357-63. [Content Brief]

[2]. Gerber S, et al. Recessive and Dominant De Novo ITPR1 Mutations Cause Gillespie Syndrome. Am J Hum Genet. 2016 May 5;98(5):971-980. [Content Brief]

IP3 Receptor Antibody (YA1596) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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