FANCD2 Antibody (YA3102)

Cat. No.: HY-P83357: Data Sheet COA
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FANCD2 Antibody (YA3102) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FANCD2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
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10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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FANCD2 Antibody (YA3102) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

FANCD2 Antibody (YA3102) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FANCD2.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 164 kDa;

Observed band size: 164 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9BXW9

Gene ID

2177 [NCBI]

Immunogen

A synthesized peptide derived from human FANCD2 aa200-255/1451.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:50

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB IHC-P ICC

Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using FANCD2 Antibody (HY-P83357) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse testicles tissue using FANCD2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse testicles tissue using FANCD2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunocytochemistry analysis of Hela cells labeling FANCD2 Antibody (HY-P83357) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with FANCD2 Antibody (HY-P83357) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of Hela cells labeling FANCD2 Antibody (HY-P83357) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with FANCD2 Antibody (HY-P83357) at 1/200 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Required for maintenance of chromosomal stability (PubMed:11239453, PubMed:14517836). Promotes accurate and efficient pairing of homologs during meiosis (PubMed:14517836). Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing (PubMed:15671039, PubMed:15650050, PubMed:30335751, PubMed:36385258). The FANCI-FANCD2 complex binds and scans double-stranded DNA (dsDNA) for DNA damage; this complex stalls at DNA junctions between double-stranded DNA and single-stranded DNA (By similarity). May participate in S phase and G2 phase checkpoint activation upon DNA damage (PubMed:15377654). Plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress (PubMed:15454491, PubMed:15661754). Required for the targeting, or stabilization, of BLM to non-centromeric abnormal structures induced by replicative stress (PubMed:15661754, PubMed:19465921). Promotes BRCA2/FANCD1 loading onto damaged chromatin (PubMed:11239454, PubMed:12239151, PubMed:12086603, PubMed:15115758, PubMed:15199141, PubMed:15671039, PubMed:18212739). May also be involved in B-cell immunoglobulin isotype switching

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:Highly expressed in germinal center cells of the spleen, tonsil, and reactive lymph nodes, and in the proliferating basal layer of squamous epithelium of tonsil, esophagus, oropharynx, larynx and cervix. Expressed in cytotrophoblastic cells of the placenta and exocrine cells of the pancreas (at protein level) . Highly expressed in testis, where expression is restricted to maturing spermatocytes

Isoforms & Post-Translational Modification：Q9BXW9 has 4 isomers: Q9BXW9-2: 164128 Da (predicted); Q9BXW9-1: 166462 Da (predicted); Q9BXW9-3: 140737 Da (predicted); Q9BXW9-4: 27501 Da (predicted).
Monoubiquitinated on Lys-561 during S phase and upon genotoxic stress by FANCL in complex with E2 ligases UBE2T or UBE2W (isoform 1 and isoform 2) (PubMed:11239454, PubMed:12973351, PubMed:19111657, PubMed:20603015, PubMed:20603016, PubMed:20603073, PubMed:36385258). Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed (PubMed:15694335, PubMed:36385258). Monoubiquitination requires the joint intervention of the FANC core complex, including FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, and FANCM, and proteins involved in cell cycle checkpoints and DNA repair, including RPA1, ATR, CHEK1 and BRCA1, and is mediated by FANCL/PHF9 (PubMed:12973351). Monoubiquitination prevents DNA release from the FANCI-FANCD2 complex (By similarity). FANCD2 is only ubiquitinated in the FANCI-FANCD2 complex and the monoubiquitination of FANCD2 is promoted by phosphorylation of FANCI (By similarity). Ubiquitination is required for binding to chromatin, interaction with BRCA1, BRCA2 and MTMR15/FAN1, DNA repair, and normal cell cycle progression, but not for phosphorylation on Ser-222 or interaction with MEN1 (PubMed:12086603, PubMed:15650050);Phosphorylated in response to various genotoxic stresses by ATM and/or ATR (PubMed:14988723, PubMed:15314022). Upon ionizing radiation, phosphorylated by ATM on Ser-222 and Ser-1404 (PubMed:12086603). Phosphorylation on Ser-222 is required for S-phase checkpoint activation, but not for ubiquitination, foci formation, or DNA repair (PubMed:12086603, PubMed:15650050). In contrast, phosphorylation by ATR on other sites may be required for ubiquitination and foci formation

Subunit：Homodimer; cannot be ubiquitinated and does not bind DNA (By similarity). Part of a FANCI-FANCD2 heterodimeric complex that binds and scans dsDNA for DNA damage (PubMed:36385258). Interacts directly with FANCE and FANCI (PubMed:12093742, PubMed:12649160, PubMed:17412408, PubMed:17460694). Interacts with USP1 and MEN1 (PubMed:12874027, PubMed:15694335). The ubiquitinated form specifically interacts with BRCA1 and BLM (PubMed:11239454, PubMed:15257300). Both the nonubiquitinated and the monoubiquitinated forms interact with BRCA2; this interaction is mediated by phosphorylated FANCG and the complex also includes XCCR3 (PubMed:15115758, PubMed:15199141, PubMed:18212739). The ubiquitinated form specifically interacts with MTMR15/FAN1 (via UBZ-type zinc finger), leading to recruit MTMR15/FAN1 to sites of DNA damage (PubMed:20603015, PubMed:20603016, PubMed:20603073). Interacts with DCLRE1B/Apollo (PubMed:18469862). Interacts with POLN (PubMed:19995904). Interacts with UHRF1 and UHRF2; these interactions promote FANCD2 activation (PubMed:30335751)

RRID

AB_3105470

Database

Entrez Gene: 2177 Human ; 211651 Mouse ; 312641 Rat

SwissProt: Q9BXW9 Human ; Q80V62 Mouse ; Q6IV68 Rat

OMIM: 227646 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

FA D2; FA4; FAC D2; FACD 2; FACD; FACD2; FAD; FAD2; FANCD 2; FANCD; FANCD2; Fanconi anemia group D2 protein; FLJ23826; Protein FACD2; Type 4 Fanconi pancytopenia

Documentation

Select Batch:

Data Sheet (235 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

FANCD2 Antibody (YA3102) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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FANCD2 Antibody (YA3102)

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FANCD2 Antibody (YA3102) Related Classifications

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