5-HT2A Antibody
(Synonyms: HTR2, HTR2A, 5-hydroxytryptamine receptor 2A, 5-HT-2, 5-HT-2A, Serotonin receptor 2A)
Based on 1 Customer Validation
5-HT2A Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to 5-HT2A.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat, Monkey, Pig, Bovine
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Formulation:
Supplied in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:50-100 |
Product Details
5-HT2A Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to 5-HT2A.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat, Monkey, Pig, Bovine
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Observed Molecular WeightObserved band size: 52 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 52 kDa
Synthetic peptide corresponding to the N-term region of human 5-HT2A.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
5-HT2A is a G protein-coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. HTR2A is coupled to G(q)/G(11) G alpha proteins and activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers that modulate the activity of phosphatidylinositol 3-kinase and promote the release of Ca(2+) ions from intracellular stores, respectively. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction (By similarity)[1][2][3][4][5][6][7][8][9].
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Subcellular Localization
Cell membrane; Cell projection, dendrite; Cell projection, axon; Cytoplasmic vesicle; Membrane, caveola; Presynapse
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Expression
Tissue_Specificity: Detected in brain cortex (at protein level). Detected in blood platelets. -
Isoforms & Post-Translational Modification
5-HT2A has 2 isoforms, P28223-1: amino acid length is 471, molecular weight is 52603 Da (predicted); P28223-2: amino acid length is 387, molecular weight is 43940 Da (predicted).
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Subunit
Interacts (via C-terminus) with MPDZ and PATJ.
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SwissProt ID
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Synonyms
HTR2, HTR2A, 5-hydroxytryptamine receptor 2A, 5-HT-2, 5-HT-2A, Serotonin receptor 2A
Documentation
References
[1]. Stam NJ, et al. Genomic organization, coding sequence and functional expression of human 5-HT2 and 5-HT1A receptor genes. Eur J Pharmacol. 1992 Oct 1;227(2):153-62. [Content Brief]
[2]. Cussac D, et al. Agonist-directed trafficking of signalling at serotonin 5-HT2A, 5-HT2B and 5-HT2C-VSV receptors mediated Gq/11 activation and calcium mobilisation in CHO cells. Eur J Pharmacol. 2008 Oct 10;594(1-3):32-8. [Content Brief]
[3]. Knauer CS, et al. Pharmacological characterization of mitogen-activated protein kinase activation by recombinant human 5-HT2C, 5-HT2A, and 5-HT2B receptors. Naunyn Schmiedebergs Arch Pharmacol. 2009 May;379(5):461-71. [Content Brief]
[4]. Albizu L, et al. Functional crosstalk and heteromerization of serotonin 5-HT2A and dopamine D2 receptors. Neuropharmacology. 2011 Sep;61(4):770-7. [Content Brief]
[5]. Delille HK, et al. Heterocomplex formation of 5-HT2A-mGlu2 and its relevance for cellular signaling cascades. Neuropharmacology. 2012 Jun;62(7):2184-91. [Content Brief]
[6]. Cao D, et al. Structure-based discovery of nonhallucinogenic psychedelic analogs. Science. 2022 Jan 28;375(6579):403-411. [Content Brief]
[7]. Chen Z, et al. Flexible scaffold-based cheminformatics approach for polypharmacological drug design. Cell. 2024 Apr 25;187(9):2194-2208.e22. [Content Brief]
[8]. Wacker D, et al. Crystal Structure of an LSD-Bound Human Serotonin Receptor. Cell. 2017 Jan 26;168(3):377-389.e12. [Content Brief]
[9]. González-Maeso J, et al. Identification of a serotonin/glutamate receptor complex implicated in psychosis. Nature. 2008 Mar 6;452(7183):93-7. [Content Brief]
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