Tat SF1 Antibody (YA2886)
(Synonyms: HTATSF1; HTSF1; TAT SF1)Tat SF1 Antibody (YA2886) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Tat SF1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, IP
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Reactivity :
Human, Mouse
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Formulation:
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:50-1:200 | 1:50 |
Product Details
Tat SF1 Antibody (YA2886) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Tat SF1.
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Host Rabbit
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Clonality Recombinant, Monoclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 140 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 86 kDa
Entrez Gene: 27336 Human ; 72459 Mouse ;
SwissProt: O43719 Human ; Q8BGC0 Mouse ;
OMIM: 300346 Human
A synthesized peptide derived from human HTSF1 aa15-70/755.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Tat SF1 component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, HTATSF1 is required to stabilize the branchpoint-interacting stem loop. HTATSF1 is displaced from the 17S U2 SnRNP complex before the stable addition of the 17S U2 SnRNP complex to the spliceosome, destabilizing the branchpoint-interacting stem loop and allowing to probe intron branch site sequences. Also acts as a regulator of transcriptional elongation, possibly by mediating the reciprocal stimulatory effect of splicing on transcriptional elongation. Involved in double-strand break (DSB) repair via homologous recombination in S-phase by promoting the recruitment of TOPBP1 to DNA damage sites. Mechanistically, HTATSF1 is (1) recruited to DNA damage sites in S-phase via interaction with poly-ADP-ribosylated RPA1 and (2) phosphorylated by CK2, promoting recruitment of TOPBP1, thereby facilitating RAD51 nucleofilaments formation and RPA displacement, followed by homologous recombination; (Microbial infection) In case of infection by HIV-1, it is up-regulated by the HIV-1 proteins NEF and gp120, acts as a cofactor required for the Tat-enhanced transcription of the virus[1][2][3][4][5][6][7].
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Subcellular Localization
Nucleus; Chromosome
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Expression
Tissue_specificity:Widely expressed -
Subunit
Component of the 17S U2 SnRNP complex, a ribonucleoprotein complex that contains small nuclear RNA (snRNA) U2 and a number of specific proteins (PubMed:11780068, PubMed:30567737, PubMed:32494006, PubMed:34822310, PubMed:9710584). Within the 17S U2 SnRNP complex, interacts (via UHM region) directly with SF3B1 (PubMed:30567737). Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin (PubMed:10393184, PubMed:10454543, PubMed:10913173, PubMed:11780068, PubMed:9649438). Interacts with GTF2F2/RAP30 and POLR2A (PubMed:10454543). Interacts with TCERG1/CA150 (PubMed:15485897). Interacts with (poly-ADP-ribosylated) RPA1; promoting HTATSF1 recruitment to DNA damage sites (PubMed:35597237). Interacts (when phosphorylated) with TOPBP1; promoting recruitment of TOPBP1 to DNA damage sites during S-phase (PubMed:35597237)
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SwissProt ID
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Synonyms
HTATSF1; HTSF1; TAT SF1
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Research Field
Microbiology
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