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Tankyrase 2 Antibody (YA6817)

(Synonyms: ADP-ribosyltransferase diphtheria toxin-like 6 antibody; ARTD6 antibody; PARP 5b antibody; PARP 5c antibody; PARP-5b antibody; PARP-5c antibody; PARP5B antibody; PARP5C antibody; pART6 antibody; Poly [ADP ribose] polymerase 5B antibody; ADP-ribosyltransferase diphtheria toxin-like 6 antibody; ARTD6 antibody; PARP 5b antibody; PARP 5c antibody)
Customer Review

Based on 1 Customer Validation

Tankyrase 2 Antibody (YA6817) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Tankyrase 2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Tankyrase 2 Antibody (YA6817)
  • Experimental Validation Results for Tankyrase 2 Antibody (YA6817)
  • Tankyrase 2 Antibody (YA6817) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000 1:250

Product Details

Description

Tankyrase 2 Antibody (YA6817) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Tankyrase 2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 127 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 127 kDa
Immunogen

Recombinant protein within human Tankyrase 2 aa 451-700/1,166.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719267

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Tankyrase 2 Antibody (YA6817)
    Immunocytochemistry analysis of A431 cells labeling Tankyrase 2 with Tankyrase 2 Antibody (YA6817) (HY-P87124) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Tankyrase 2 Antibody (YA6817) ((HY-P87124) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Tankyrase 2 Antibody (YA6817)
    Immunocytochemistry analysis of A431 cells labeling Tankyrase 2 with Tankyrase 2 Antibody (YA6817) (HY-P87124) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Tankyrase 2 Antibody (YA6817) ((HY-P87124) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Tankyrase 2 is a Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates poly-ADP-ribosylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates poly-ADP-ribosylation of TERF1, thereby contributing to the regulation of telomere length. Stimulates 26S proteasome activity[1][2][3][4][5][6].

  • Subcellular Localization

    Cytoplasm,Golgi apparatus membrane,Nucleus,Chromosome, telomere

  • Expression


    Tissue_Specificity: Highly expressed in placenta, skeletal muscle, liver, brain, kidney, heart, thymus, spinal cord, lung, peripheral blood leukocytes, pancreas, lymph nodes, spleen, prostate, testis, ovary, small intestine, colon, mammary gland, breast and breast carcinoma, and in common-type meningioma. Highly expressed in fetal liver, heart and brain

  • Isoforms & Post-Translational Modification

    Q9H2K2: 1166 amino acids, molecular weight 126918 Da.

  • Subunit

    Oligomerizes and associates with TNKS

  • SwissProt ID

    Q9H2K2

  • Gene ID
    80351 [NCBI]
  • Synonyms

    ADP-ribosyltransferase diphtheria toxin-like 6 antibody; ARTD6 antibody; PARP 5b antibody; PARP 5c antibody; PARP-5b antibody; PARP-5c antibody; PARP5B antibody; PARP5C antibody; pART6 antibody; Poly [ADP ribose] polymerase 5B antibody; ADP-ribosyltransferase diphtheria toxin-like 6 antibody; ARTD6 antibody; PARP 5b antibody; PARP 5c antibody

Tankyrase 2 Antibody (YA6817) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling Tankyrase 2 with Tankyrase 2 Antibody (YA6817) (HY-P87124) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Tankyrase 2 Antibody (YA6817) ((HY-P87124) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling Tankyrase 2 with Tankyrase 2 Antibody (YA6817) (HY-P87124) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Tankyrase 2 Antibody (YA6817) ((HY-P87124) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Tankyrase 2 Antibody (YA6817) Product Image
    1/16
  • Experimental Validation Results for Tankyrase 2 Antibody (YA6817)
  • Experimental Validation Results for Tankyrase 2 Antibody (YA6817)
  • Tankyrase 2 Antibody (YA6817) Product Image
  • Experimental Validation Results for Tankyrase 2 Antibody (YA6817)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling Tankyrase 2 with Tankyrase 2 Antibody (YA6817) (HY-P87124) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Tankyrase 2 Antibody (YA6817) ((HY-P87124) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Tankyrase 2 Antibody (YA6817)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling Tankyrase 2 with Tankyrase 2 Antibody (YA6817) (HY-P87124) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Tankyrase 2 Antibody (YA6817) ((HY-P87124) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Tankyrase 2 Antibody (YA6817)
  • Experimental Validation Results for Tankyrase 2 Antibody (YA6817)
  • Tankyrase 2 Antibody (YA6817) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A431 cells labeling Tankyrase 2 with Tankyrase 2 Antibody (YA6817) (HY-P87124) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Tankyrase 2 Antibody (YA6817) ((HY-P87124) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Tankyrase 2 Antibody (YA6817) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A431 cells labeling Tankyrase 2 with Tankyrase 2 Antibody (YA6817) (HY-P87124) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Tankyrase 2 Antibody (YA6817) ((HY-P87124) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Tankyrase 2 Antibody (YA6817) Product Image ICC
  • Tankyrase 2 Antibody (YA6817) Product Image ICC