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Elk-1 Antibody (YA7598)

Customer Review

Based on 1 Customer Validation

Elk-1 Antibody (YA7598) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Elk-1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG,Kappa

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
WB
  • Experimental Validation Results for Elk-1 Antibody (YA7598)
  • Experimental Validation Results for Elk-1 Antibody (YA7598)
  • Experimental Validation Results for Elk-1 Antibody (YA7598)
  • Elk-1 Antibody (YA7598) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:200-1:1000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

Elk-1 Antibody (YA7598) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Elk-1.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 62 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 62 kDa
Immunogen

The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG,Kappa

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Elk-1 Antibody (YA7598)
    Western blot analysis was performed on protein extracts (30 μg) from Rat heart (lane 2), Mouse heart (lane 3), Rat brain (lane 4), and Mouse brain (lane 5) using Elk-1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Elk-1 Antibody (YA7598)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、K562 (lane 2(20μg)) and Mouse brain (lane 3(20μg) using Elk-1 Antibody (HY-P87913) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/4000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Elk-1 Antibody (YA7598)
    Western blot analysis of extracts from Mouse brain (lane 1(20μg)) and Rat brain (lane 2(20μg)) using Elk-1 Antibody (HY-P87913) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Elk-1 transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK and MAPK-signaling pathways stimulation[1][2][3].

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_Specificity: Lung and testis

  • Isoforms & Post-Translational Modification

    P19419 has two isomers: P19419-1: 44888 Da (predicted); P19419-2: 11217 Da (predicted).
    Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity丨On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1

  • Subunit

    Interacts in its sumoylated form with PIAS2/PIASX which enhances its transcriptional activator activity (PubMed:15920481)

  • SwissProt ID

    P19419

Elk-1 Antibody (YA7598) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from Rat heart (lane 2), Mouse heart (lane 3), Rat brain (lane 4), and Mouse brain (lane 5) using Elk-1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、K562 (lane 2(20μg)) and Mouse brain (lane 3(20μg) using Elk-1 Antibody (HY-P87913) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/4000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Mouse brain (lane 1(20μg)) and Rat brain (lane 2(20μg)) using Elk-1 Antibody (HY-P87913) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Elk-1 Antibody (YA7598) Product Image
    1/16
  • Experimental Validation Results for Elk-1 Antibody (YA7598)
  • Experimental Validation Results for Elk-1 Antibody (YA7598)
  • Experimental Validation Results for Elk-1 Antibody (YA7598)
  • Elk-1 Antibody (YA7598) Product Image
  • Experimental Validation Results for Elk-1 Antibody (YA7598)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from Rat heart (lane 2), Mouse heart (lane 3), Rat brain (lane 4), and Mouse brain (lane 5) using Elk-1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Elk-1 Antibody (YA7598)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、K562 (lane 2(20μg)) and Mouse brain (lane 3(20μg) using Elk-1 Antibody (HY-P87913) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/4000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Elk-1 Antibody (YA7598)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Mouse brain (lane 1(20μg)) and Rat brain (lane 2(20μg)) using Elk-1 Antibody (HY-P87913) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Elk-1 Antibody (YA7598)
  • Experimental Validation Results for Elk-1 Antibody (YA7598)
  • Experimental Validation Results for Elk-1 Antibody (YA7598)
  • Elk-1 Antibody (YA7598) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (30 μg) from Rat heart (lane 2), Mouse heart (lane 3), Rat brain (lane 4), and Mouse brain (lane 5) using Elk-1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Elk-1 Antibody (YA7598) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、K562 (lane 2(20μg)) and Mouse brain (lane 3(20μg) using Elk-1 Antibody (HY-P87913) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/4000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Elk-1 Antibody (YA7598) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Mouse brain (lane 1(20μg)) and Rat brain (lane 2(20μg)) using Elk-1 Antibody (HY-P87913) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Elk-1 Antibody (YA7598) Product Image WB
  • Elk-1 Antibody (YA7598) Product Image WB
  • Elk-1 Antibody (YA7598) Product Image WB