Cofilin Antibody (YA7568)

Cat. No.: HY-P87883: Data Sheet User Guide for Antibodies
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Cofilin Antibody (YA7568) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Cofilin.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Cofilin Antibody (YA7568) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Cofilin.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 19 kDa;

Observed band size: 19 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P23528

Immunogen

The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:20000-1:50000
WB WB: Western Blot	1:2000-1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000

Sensitivity	Endogenous	Purity	Protein A affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG,Kappa

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed:21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity)

Subcellular Localization：Nucleus matrix,Cytoplasm, cytoskeleton,Cell projection, ruffle membrane,Cell projection, lamellipodium membrane,Cell projection, lamellipodium,Cell projection, growth cone,Cell projection, axon

Expression：
Tissue_Specificity: Widely distributed in various tissues
Induction: Up-regulated in response to enterovirus 71 (EV71) infection (at protein level)

Isoforms & Post-Translational Modification：P23528: 166 amino acids, molecular weight 18502 Da.

Subunit：Can bind G- and F-actin in a 1:1 ratio of cofilin to actin (PubMed:11812157)

Documentation

User Guide for Antibodies (1077 KB)