hnRNP K Antibody (YA7652)
(Synonyms: HNRPK, HNRNPK, Heterogeneous nuclear ribonucleoprotein K, hnRNP K, Transformation up-regulated nuclear protein, TUNP)hnRNP K Antibody (YA7652) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to hnRNP K.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IHC-F, IF-Tissue, FC, ICC/IF, IP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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FC
FC: Flow Cytometry
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:100-200 | 1:100-200 | 1:50-200 | 1:50-100 | 1:50-200 | 1:20-50 |
Product Details
hnRNP K Antibody (YA7652) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to hnRNP K.
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Host Rabbit
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Clonality Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 60 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 51 kDa
A synthesized peptide derived from human hnRNP K
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a multifunctional RNA- and DNA-binding protein that regulates gene expression at transcriptional and translational levels[1][2]. Mechanistically, hnRNP K interacts with signal transducers and chromatin modifiers to modulate mRNA splicing, stability, and translation, and undergoes dynamic subcellular localization during cell cycle progression[3][4][5]. In hepatocytes and vascular smooth muscle cells, hnRNP K translocates from the nucleus to the cytoplasm in response to proliferation signals, indicating a role in cell cycle-dependent gene regulation[3][4]. Compared with other hnRNP isoforms such as hnRNP A2, hnRNP K is specifically recruited to inducible gene loci and stress granules, suggesting isoform-specific functions in transcriptional activation and stress response[6][1]. In disease models, hnRNP K overexpression promotes tumor proliferation, metastasis, and survival, whereas RNA interference-mediated downregulation suppresses growth and induces apoptosis in lung and melanoma cancer cells[7][8][9][10]. Viral infections, including dengue, Junín, and hepatitis C, exploit hnRNP K cytoplasmic localization to enhance viral replication, highlighting its role as a host factor[11][12][6]. Pharmacological or genetic targeting of hnRNP K, including inhibition of MAPK/ERK signaling, sensitizes cancer cells to apoptosis and DNA damage, underscoring its potential as a therapeutic target[8][9]. Structurally, hnRNP K contains three KH domains and RGG motifs that mediate combinatorial nucleic acid binding, and methylation does not significantly alter its RNA-binding affinity but may influence interaction specificity[2][13].
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Subcellular Localization
Cytoplasm,Nucleus, nucleoplasm,Cell projection, podosome
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Expression
Induction: By DNA damage, including ionizing radiations and phleomycin treatment or UV irradiation. This induction requires ATM kinase activity (ionizing radiations and phleomycin) or ATR activity (UV irradiation). Up-regulation is due to protein stabilization. Constitutive protein levels are controlled by MDM2-mediated ubiquitination and degradation via the proteasome pathway
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Isoforms & Post-Translational Modification
P61978 has three isomers: P61978-1: 50976 Da (predicted); P61978-2: 51028 Da (predicted); P61978-3: 48562 Da (predicted).
Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine丨Sumoylated by CBX4丨Ubiquitinated by MDM2丨O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner -
Subunit
Identified in the spliceosome C complex (PubMed:11991638)
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SwissProt ID
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Synonyms
HNRPK, HNRNPK, Heterogeneous nuclear ribonucleoprotein K, hnRNP K, Transformation up-regulated nuclear protein, TUNP
Documentation
References
[1]. Guo Y, et al. Heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a tissue biomarker for detection of early hepatocellular carcinoma in patients with cirrhosis. J Hematol Oncol. 2012 Jul 3;5:37. [Content Brief]
[2]. Chang CJ, et al. The heterogeneous nuclear ribonucleoprotein K (hnRNP K) interacts with dengue virus core protein. DNA Cell Biol. 2001 Sep;20(9):569-77. [Content Brief]
[3]. Ostrowski J, et al. Nuclear shift of hnRNP K protein in neoplasms and other states of enhanced cell proliferation. Br J Cancer. 2003 Oct 20;89(8):1493-501. [Content Brief]
[4]. Gao R, et al. Heterogeneous nuclear ribonucleoprotein K (hnRNP-K) promotes tumor metastasis by induction of genes involved in extracellular matrix, cell movement, and angiogenesis. J Biol Chem. 2013 May 24;288(21):15046-56. [Content Brief]
[5]. Fan B, et al. A human proteome microarray identifies that the heterogeneous nuclear ribonucleoprotein K (hnRNP K) recognizes the 5' terminal sequence of the hepatitis C virus RNA. Mol Cell Proteomics. 2014 Jan;13(1):84-92. [Content Brief]
[6]. Laury-Kleintop LD, et al. Compartmentalization of hnRNP-K during cell cycle progression and its interaction with calponin in the cytoplasm. J Cell Biochem. 2005 Aug 1;95(5):1042-56. [Content Brief]
[7]. Brunetti JE, et al. The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a host factor required for dengue virus and Junín virus multiplication. Virus Res. 2015 May 4;203:84-91. [Content Brief]
[8]. Eder S, et al. Radiosensitization and downregulation of heterogeneous nuclear ribonucleoprotein K (hnRNP K) upon inhibition of mitogen/extracellular signal-regulated kinase (MEK) in malignant melanoma cells. Oncotarget. 2015 Jul 10;6(19):17178-91. [Content Brief]
[9]. Fukuda T, et al. hnRNP K interacts with RNA binding motif protein 42 and functions in the maintenance of cellular ATP level during stress conditions. Genes Cells. 2009 Feb;14(2):113-28. [Content Brief]
[10]. Tang F, et al. Downregulation of hnRNP K by RNAi inhibits growth of human lung carcinoma cells. Oncol Lett. 2014 Apr;7(4):1073-1077. [Content Brief]
[11]. Stone SN, et al. Naloxone-Prescribing Practices in a Freestanding Rehabilitation Hospital. Am J Phys Med Rehabil. 2024 Feb 1;103(2):105-109. [Content Brief]
[12]. Ostrowski J, et al. Transient recruitment of the hnRNP K protein to inducibly transcribed gene loci. Nucleic Acids Res. 2003 Jul 15;31(14):3954-62. [Content Brief]
[13]. van Domselaar R, et al. All human granzymes target hnRNP K that is essential for tumor cell viability. J Biol Chem. 2012 Jun 29;287(27):22854-64. [Content Brief]
[14]. Moritz B, et al. Biophysical and biochemical analysis of hnRNP K: arginine methylation, reversible aggregation and combinatorial binding to nucleic acids. Biol Chem. 2014 Jul;395(7-8):837-53. [Content Brief]
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