hnRNP K Antibody (YA7652)

Cat. No.: HY-P87967: Data Sheet User Guide for Antibodies
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hnRNP K Antibody (YA7652) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to hnRNP K.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

hnRNP K Antibody (YA7652) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to hnRNP K.

Host

Rabbit

Clonality

Recombinant

Molecular Weight

Predicted band size: 51 kDa;

Observed band size: 60 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P61978

Immunogen

A synthesized peptide derived from human hnRNP K

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-200
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-200
FC FC: Flow Cytometry	1:50-100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200
IP IP: Immunoprecipitation	1:20-50

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest. As part of a ribonucleoprotein complex composed at least of ZNF827, HNRNPL and the circular RNA circZNF827 that nucleates the complex on chromatin, may negatively regulate the transcription of genes involved in neuronal differentiation (PubMed:33174841)

Subcellular Localization：Cytoplasm,Nucleus, nucleoplasm,Cell projection, podosome

Expression：Induction: By DNA damage, including ionizing radiations and phleomycin treatment or UV irradiation. This induction requires ATM kinase activity (ionizing radiations and phleomycin) or ATR activity (UV irradiation). Up-regulation is due to protein stabilization. Constitutive protein levels are controlled by MDM2-mediated ubiquitination and degradation via the proteasome pathway

Isoforms & Post-Translational Modification：P61978 has three isomers: P61978-1: 50976 Da (predicted); P61978-2: 51028 Da (predicted); P61978-3: 48562 Da (predicted).
Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine丨Sumoylated by CBX4丨Ubiquitinated by MDM2丨O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner

Subunit：Identified in the spliceosome C complex (PubMed:11991638)

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/a-431-cell.html [Content Brief]