TRPM8 Antibody
(Synonyms: LTRPC6, TRPP8, TRPM8, Transient receptor potential cation channel subfamily M member 8, Long transient receptor potential channel 6, Transient receptor potential p8, LTrpC-6, LTrpC6, Trp-p8)
Based on 1 Customer Validation
TRPM8 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to TRPM8.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:50-100 | 1:50-200 |
Product Details
TRPM8 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to TRPM8.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 130 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 127 kDa
Synthetic peptide corresponding to the center region of human TRPM8.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
TRPM8 is a Non-selective ion channel permeable to monovalent and divalent cations, including Na(+), K(+), and Ca(2+), with higher permeability for Ca(2+). Activated by multiple factors, such as temperature, voltage, pressure, and changes in osmolality. Activated by cool temperatures (<23-28 degrees Celsius) and by chemical ligands evoking a sensation of coolness, such as menthol and icilin therefore plays a central role in the detection of environmental cold temperatures. TRPM8 is a voltage-dependent channel; its activation by cold or chemical ligands shifts its voltage thresholds towards physiological membrane potentials, leading to the opening of the channel. In addition to its critical role in temperature sensing, regulates basal tear secretion by sensing evaporation-induced cooling and changes in osmolality (By similarity). May play a role in prostate cancer cell migration[1][2][3][4][5].
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Subcellular Localization
Cell membrane; Membrane raft; Endoplasmic reticulum membrane
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Expression
Tissue_Specificity: Expressed in prostate. Also expressed in prostate tumors and in non-prostatic primary tumors such as colon, lung, breast and skin tumors. -
Isoforms & Post-Translational Modification
TRPM8 has 4 isoforms, Q7Z2W7-1: amino acid length is 1104, molecular weight is 127685 Da (predicted); Q7Z2W7-2: amino acid length is 54, molecular weight is 5940 Da (predicted); Q7Z2W7-3: amino acid length is 165, molecular weight is 18655 Da (predicted); Q7Z2W7-4: amino acid length is 682, molecular weight is 79096 Da (predicted).TRPM8 存在 4 个异构体，Q7Z2W7-1：氨基酸个数为 1104 个，分子量为 127685 Da (预测)；Q7Z2W7-2：氨基酸个数为 54 个，分子量为 5940 Da (预测)；Q7Z2W7-3：氨基酸个数为 165 个，分子量为 18655 Da (预测)；Q7Z2W7-4：氨基酸个数为 682 个，分子量为 79096 Da (预测)。
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Subunit
Homotetramer.
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SwissProt ID
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Synonyms
LTRPC6, TRPP8, TRPM8, Transient receptor potential cation channel subfamily M member 8, Long transient receptor potential channel 6, Transient receptor potential p8, LTrpC-6, LTrpC6, Trp-p8
Documentation
[1]. Voets T, et al. The principle of temperature-dependent gating in cold- and heat-sensitive TRP channels. Nature. 2004 Aug 12;430(7001):748-54. [Content Brief]
[2]. Rohács T, et al. PI(4,5)P2 regulates the activation and desensitization of TRPM8 channels through the TRP domain. Nat Neurosci. 2005 May;8(5):626-34. [Content Brief]
[3]. Thebault S, et al. Novel role of cold/menthol-sensitive transient receptor potential melastatine family member 8 (TRPM8) in the activation of store-operated channels in LNCaP human prostate cancer epithelial cells. J Biol Chem. 2005 Nov 25;280(47):39423-35. [Content Brief]
[4]. Gkika D, et al. TRP channel-associated factors are a novel protein family that regulates TRPM8 trafficking and activity. J Cell Biol. 2015 Jan 5;208(1):89-107. [Content Brief]
[5]. Palchevskyi S, et al. Structure of human TRPM8 channel. Commun Biol. 2023 Oct 19;6(1):1065. [Content Brief]
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