alpha Tubulin (acetyl K40) Antibody (YA626)

Cat. No.: HY-P80485: Data Sheet COA
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alpha Tubulin (acetyl K40) Antibody (YA626) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to alpha Tubulin (acetyl K40).

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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alpha Tubulin (acetyl K40) Antibody (YA626) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

alpha Tubulin (acetyl K40) Antibody (YA626) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to alpha Tubulin (acetyl K40).

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 50 kDa；

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P68363

Gene ID

10376 [NCBI]

Immunogen

Synthetic peptide corresponding to Human alpha Tubulin.AA range:30-45.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Acetylated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from C6 (lane 2(20μg) , Hela(lane 3(20μg) and NIH3T3(lane 4(20μg) using alpha Tubulin (acetyl K40) (HY-P80485) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers (PubMed:38305685, PubMed:34996871, PubMed:38609661). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:38305685, PubMed:34996871, PubMed:38609661). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871, PubMed:38609661)

Subcellular Localization：Cytoplasm, cytoskeleton

Expression：
Induction:Autoregulated by feedback control of mRNA degradation (PubMed:31727855) . In excess of soluble tubulin, TTC5/STRAP cofactor triggers cotranslation degradation of tubulin mRNA (PubMed:31727855)

Isoforms & Post-Translational Modification：P68363 has 2 isomers: P68363-1: 50152 Da (predicted); P68363-2: 37218 Da (predicted).
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Glutamylation is also involved in cilia motility (By similarity);Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility. Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally;Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly;Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability;Nitration of Tyr-451 is irreversible and interferes with normal dynein intracellular distribution;Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (MATCAP1/KIAA0895L, VASH1 or VASH2) and tubulin tyrosine ligase (TTL), respectively;Tyrosination promotes microtubule interaction with CAP-Gly domain-containing proteins such as CLIP1, CLIP2 and DCTN1 (By similarity). Tyrosination regulates the initiation of dynein-dynactin motility via interaction with DCTN1, which brings the dynein-dynactin complex into contact with microtubules (PubMed:26972003). In neurons, tyrosinated tubulins mediate the initiation of retrograde vesicle transport (By similarity);Detyrosination is involved in metaphase plate congression by guiding chromosomes during mitosis: detyrosination promotes interaction with CENPE, promoting pole-proximal transport of chromosomes toward the equator (PubMed:25908662). Detyrosination increases microtubules-dependent mechanotransduction in dystrophic cardiac and skeletal muscle. In cardiomyocytes, detyrosinated microtubules are required to resist to contractile compression during contraction: detyrosination promotes association with desmin (DES) at force-generating sarcomeres, leading to buckled microtubules and mechanical resistance to contraction (By similarity)

Subunit：Heterodimer of alpha- and beta-tubulin (PubMed:17563362, PubMed:34996871, PubMed:35482892, PubMed:38305685, PubMed:38609661). A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM (PubMed:34996871, PubMed:35482892). Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity (PubMed:34996871, PubMed:35482892, PubMed:38305685, PubMed:38609661). Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells (PubMed:34996871, PubMed:35482892, PubMed:38305685, PubMed:38609661). Interacts with gamma-tubulin; the interaction allows microtubules to nucleate from the gamma-tubulin ring complex (gTuRC) (PubMed:38305685, PubMed:38609661). Nascent microtubule interacts (via alpha-tubulin MREC motif) with TTC5/STRAP; this interaction may result in tubulin mRNA-targeted degradation (PubMed:31727855). Component of sperm flagellar doublet microtubules (By similarity)

RRID

AB_3102558

Database

Entrez Gene: 10376 Human

SwissProt: P68363 Human

OMIM: 602530 Human

Research Field

Isotype/Loading Controls

Documentation

Select Batch:

Data Sheet (232 KB) SDS (313 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

alpha Tubulin (acetyl K40) Antibody (YA626) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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alpha Tubulin (acetyl K40) Antibody (YA626)

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