alpha Tubulin (acetyl K40) Antibody (YA626)
(Synonyms: Tubulin alpha-1B chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain, TUBA1B)
Based on 1 Customer Validation
alpha Tubulin (acetyl K40) Antibody (YA626) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to alpha Tubulin (acetyl K40).
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human
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Formulation:
Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:1000 |
Product Details
alpha Tubulin (acetyl K40) Antibody (YA626) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to alpha Tubulin (acetyl K40).
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 55 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 50 kDa；
Synthetic peptide corresponding to Human alpha Tubulin.AA range:30-45.
Endogenous
Protein A affinity purified.
Non-conjugated
Acetylated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from C6 (lane 2(20μg) , Hela(lane 3(20μg) and NIH3T3(lane 4(20μg) using alpha Tubulin (acetyl K40) (HY-P80485) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Background
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Function
alpha Tubulin is a Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin[1][2][3].
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Subcellular Localization
Cytoplasm, cytoskeleton
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Expression
Induction:Autoregulated by feedback control of mRNA degradation (PubMed:31727855) . In excess of soluble tubulin, TTC5/STRAP cofactor triggers cotranslation degradation of tubulin mRNA (PubMed:31727855) -
Isoforms & Post-Translational Modification
P68363 has 2 isomers: P68363-1: 50152 Da (predicted); P68363-2: 37218 Da (predicted).
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Glutamylation is also involved in cilia motility (By similarity);Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility. Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally;Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly;Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability;Nitration of Tyr-451 is irreversible and interferes with normal dynein intracellular distribution;Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (MATCAP1/KIAA0895L, VASH1 or VASH2) and tubulin tyrosine ligase (TTL), respectively;Tyrosination promotes microtubule interaction with CAP-Gly domain-containing proteins such as CLIP1, CLIP2 and DCTN1 (By similarity). Tyrosination regulates the initiation of dynein-dynactin motility via interaction with DCTN1, which brings the dynein-dynactin complex into contact with microtubules (PubMed:26972003). In neurons, tyrosinated tubulins mediate the initiation of retrograde vesicle transport (By similarity);Detyrosination is involved in metaphase plate congression by guiding chromosomes during mitosis: detyrosination promotes interaction with CENPE, promoting pole-proximal transport of chromosomes toward the equator (PubMed:25908662). Detyrosination increases microtubules-dependent mechanotransduction in dystrophic cardiac and skeletal muscle. In cardiomyocytes, detyrosinated microtubules are required to resist to contractile compression during contraction: detyrosination promotes association with desmin (DES) at force-generating sarcomeres, leading to buckled microtubules and mechanical resistance to contraction (By similarity) -
Subunit
Heterodimer of alpha- and beta-tubulin (PubMed:17563362, PubMed:34996871, PubMed:35482892, PubMed:38305685, PubMed:38609661). A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM (PubMed:34996871, PubMed:35482892). Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity (PubMed:34996871, PubMed:35482892, PubMed:38305685, PubMed:38609661). Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells (PubMed:34996871, PubMed:35482892, PubMed:38305685, PubMed:38609661). Interacts with gamma-tubulin; the interaction allows microtubules to nucleate from the gamma-tubulin ring complex (gTuRC) (PubMed:38305685, PubMed:38609661). Nascent microtubule interacts (via alpha-tubulin MREC motif) with TTC5/STRAP; this interaction may result in tubulin mRNA-targeted degradation (PubMed:31727855). Component of sperm flagellar doublet microtubules (By similarity)
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SwissProt ID
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Synonyms
Tubulin alpha-1B chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain, TUBA1B
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Research Field
Isotype/Loading Controls
Documentation
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Data Sheet (260 KB)
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SDS (313 KB)
- English - EN (313 KB)
- Français - FR (313 KB)
- Deutsch - DE (313 KB)
- Norwegian - NO (313 KB)
- Español - ES (313 KB)
- Swedish - SV (313 KB)
- Italian - IT (313 KB)
- Korean - KR (313 KB)
- Portuguese - PT (313 KB)
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User Guide for Antibodies (1077 KB)
References
[1]. Brito C, et al. Transition of human γ-tubulin ring complex into a closed conformation during microtubule nucleation. Science. 2024 Feb 23;383(6685):870-876. [Content Brief]
[2]. LaFrance BJ, et al. Structural transitions in the GTP cap visualized by cryo-electron microscopy of catalytically inactive microtubules. Proc Natl Acad Sci U S A. 2022 Jan 11;119(2):. [Content Brief]
[3]. Aher A, et al. Structure of the γ-tubulin ring complex-capped microtubule. Nat Struct Mol Biol. 2024 Jul;31(7):1124-1133. [Content Brief]
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