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Pericentrin Antibody (YA6715)

(Synonyms: Centrosome Marker antibody; Ken antibody; Kendrin antibody; KIAA0402 antibody; MOPD2 antibody; PCN antibody; PCNT 2 antibody; PCNT antibody; PCNT B antibody; PCNT_HUMAN antibody; Centrosome Marker antibody; Ken antibody; Kendrin antibody; KIAA0402 antibody; MOPD2 antibody; PCN antibody; PCNT 2 antibody; PCNT antibody; PCNT B antibody; PCNT_HUMAN antibody; PCNT1 antibody; PCNT2 antibody; PCNTB antibody; PCTN2 antibody; Pericentrin 1 antibody; Pericentrin 2 antibody; Pericentrin 380 antibody; Pericentrin antibody; Pericentrin B antibody; Pericentrin-B antibody; SCKL4 antibody; )
Customer Review

Based on 1 Customer Validation

Pericentrin Antibody (YA6715) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pericentrin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Pericentrin Antibody (YA6715)
  • Pericentrin Antibody (YA6715) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000 1:100

Product Details

Description

Pericentrin Antibody (YA6715) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pericentrin.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 460 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 378 kDa
Immunogen

Recombinant protein within human aa 201-600 / 3,336.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719250

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Pericentrin Antibody (YA6715)
    Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、Hela (lane 3(20μg)) and HEK293 (lane 4(20μg)) using Pericentrin Antibody (HY-P87022) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Pericentrin is an Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome

  • Subcellular Localization

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

  • Expression


    Tissue_Specificity: Expressed in all tissues tested, including placenta, liver, kidney and thymus

  • Isoforms & Post-Translational Modification

    O95613 has two isomers: O95613-1: 378037 Da (predicted); O95613-2: 355882 Da (predicted).
    Cleaved during mitotis which leads to removal of CDK5RAP2 from the centrosome and promotes centriole disengagement and subsequent centriole separation (PubMed:22722493, PubMed:25503564)丨Ubiquitinated by TRIM43; leading to proteasomal degradation

  • Subunit

    Interacts with CHD3

  • SwissProt ID

    O95613

  • Gene ID
    5116 [NCBI]
  • Synonyms

    Centrosome Marker antibody; Ken antibody; Kendrin antibody; KIAA0402 antibody; MOPD2 antibody; PCN antibody; PCNT 2 antibody; PCNT antibody; PCNT B antibody; PCNT_HUMAN antibody; Centrosome Marker antibody; Ken antibody; Kendrin antibody; KIAA0402 antibody; MOPD2 antibody; PCN antibody; PCNT 2 antibody; PCNT antibody; PCNT B antibody; PCNT_HUMAN antibody; PCNT1 antibody; PCNT2 antibody; PCNTB antibody; PCTN2 antibody; Pericentrin 1 antibody; Pericentrin 2 antibody; Pericentrin 380 antibody; Pericentrin antibody; Pericentrin B antibody; Pericentrin-B antibody; SCKL4 antibody;

Pericentrin Antibody (YA6715) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、Hela (lane 3(20μg)) and HEK293 (lane 4(20μg)) using Pericentrin Antibody (HY-P87022) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Pericentrin Antibody (YA6715) Product Image
    1/16
  • Experimental Validation Results for Pericentrin Antibody (YA6715)
  • Pericentrin Antibody (YA6715) Product Image
  • Experimental Validation Results for Pericentrin Antibody (YA6715)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、Hela (lane 3(20μg)) and HEK293 (lane 4(20μg)) using Pericentrin Antibody (HY-P87022) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Pericentrin Antibody (YA6715)
  • Pericentrin Antibody (YA6715) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、Hela (lane 3(20μg)) and HEK293 (lane 4(20μg)) using Pericentrin Antibody (HY-P87022) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Pericentrin Antibody (YA6715) Product Image WB