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Visfatin Antibody (YA5011)

(Synonyms: NAMPT; PBEF; PBEF1; Nicotinamide phosphoribosyltransferase; NAmPRTase; Nampt; Pre-B-cell colony-enhancing factor 1; Pre-B cell-enhancing factor; Visfatin)
Customer Review

Based on 1 Customer Validation

Visfatin Antibody (YA5011) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Visfatin.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human, Rat, Mouse, Monkey, Rabbit

  • Formulation:

    Supplied in Ascitic fluid containing 0.03% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Visfatin Antibody (YA5011) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:50-1:200 1:200-1:400 1:10000

Product Details

Description

Visfatin Antibody (YA5011) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Visfatin.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Rat, Mouse, Monkey, Rabbit
  • Calculated Molecular Weight Predicted band size: 56 kDa;
Immunogen

Purified recombinant fragment of PBEF1 (aa338-479) expressed in E. Coli. The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3719022

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in Ascitic fluid containing 0.03% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Visfatin Antibody (YA5011)
    Western blot analysis of extracts from K562 (lane 2(20μg), Hela (lane 3(20μg), HL60 (lane 4(20μg) and NIH/3T3 (lane 5(20μg) using Visfatin Antibody (HY-P85319). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    Immunohistochemical analysis of paraffin-embedded human lung tissue using Visfatin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P85319, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    Immunohistochemical analysis of paraffin-embedded rat lung tissue using Visfatin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P85319, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    Immunohistochemical analysis of paraffin-embedded mouse lung tissue using Visfatin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P85319, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    Immunohistochemical analysis of paraffin-embedded human cervix tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    Immunohistochemical analysis of paraffin-embedded human bone marrow tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    Immunohistochemical analysis of paraffin-embedded human breast tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    Immunocytochemistry analysis of HCT116 cells labeling Visfatin with Visfatin antibody (HY-P85319) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Visfatin antibody (HY-P85319) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    Immunocytochemistry analysis of HCT116 cells labeling Visfatin withVisfatin antibody (HY-P85319) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Visfatin antibody (HY-P85319) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Visfatin catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression

  • Subcellular Localization

    Nucleus; Cytoplasm; Secreted

  • Expression


    Tissue_specificity:It is abundant in bone marrow, liver tissue, and muscles. It is also found in heart, placenta, lung, and kidney tissues.

  • Subunit

    Homodimer

  • SwissProt ID

    P43490

  • Gene ID
    10135 [NCBI]
  • Synonyms

    NAMPT; PBEF; PBEF1; Nicotinamide phosphoribosyltransferase; NAmPRTase; Nampt; Pre-B-cell colony-enhancing factor 1; Pre-B cell-enhancing factor; Visfatin

Visfatin Antibody (YA5011) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg), Hela (lane 3(20μg), HL60 (lane 4(20μg) and NIH/3T3 (lane 5(20μg) using Visfatin Antibody (HY-P85319). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung tissue using Visfatin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P85319, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat lung tissue using Visfatin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P85319, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse lung tissue using Visfatin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P85319, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervix tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human small intestine tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bone marrow tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HCT116 cells labeling Visfatin with Visfatin antibody (HY-P85319) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Visfatin antibody (HY-P85319) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HCT116 cells labeling Visfatin withVisfatin antibody (HY-P85319) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Visfatin antibody (HY-P85319) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Visfatin Antibody (YA5011) Product Image
    1/16
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Visfatin Antibody (YA5011) Product Image
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg), Hela (lane 3(20μg), HL60 (lane 4(20μg) and NIH/3T3 (lane 5(20μg) using Visfatin Antibody (HY-P85319). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung tissue using Visfatin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P85319, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat lung tissue using Visfatin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P85319, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse lung tissue using Visfatin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P85319, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervix tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human small intestine tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bone marrow tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HCT116 cells labeling Visfatin with Visfatin antibody (HY-P85319) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Visfatin antibody (HY-P85319) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Visfatin Antibody (YA5011)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HCT116 cells labeling Visfatin withVisfatin antibody (HY-P85319) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Visfatin antibody (HY-P85319) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Experimental Validation Results for Visfatin Antibody (YA5011)
  • Visfatin Antibody (YA5011) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 2(20μg), Hela (lane 3(20μg), HL60 (lane 4(20μg) and NIH/3T3 (lane 5(20μg) using Visfatin Antibody (HY-P85319). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Visfatin Antibody (YA5011) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung tissue using Visfatin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P85319, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Visfatin Antibody (YA5011) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat lung tissue using Visfatin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P85319, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Visfatin Antibody (YA5011) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue using Visfatin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P85319, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Visfatin Antibody (YA5011) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cervix tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Visfatin Antibody (YA5011) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Visfatin Antibody (YA5011) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Visfatin Antibody (YA5011) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Visfatin Antibody (YA5011) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human bone marrow tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Visfatin Antibody (YA5011) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast tissue using Visfatin Antibody (HY-P85319, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Visfatin Antibody (YA5011) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HCT116 cells labeling Visfatin with Visfatin antibody (HY-P85319) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Visfatin antibody (HY-P85319) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Visfatin Antibody (YA5011) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HCT116 cells labeling Visfatin withVisfatin antibody (HY-P85319) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Visfatin antibody (HY-P85319) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Visfatin Antibody (YA5011) Product Image WB
  • Visfatin Antibody (YA5011) Product Image IHC-P
  • Visfatin Antibody (YA5011) Product Image IHC-P
  • Visfatin Antibody (YA5011) Product Image IHC-P
  • Visfatin Antibody (YA5011) Product Image IHC-P
  • Visfatin Antibody (YA5011) Product Image IHC-P
  • Visfatin Antibody (YA5011) Product Image IHC-P
  • Visfatin Antibody (YA5011) Product Image IHC-P
  • Visfatin Antibody (YA5011) Product Image IHC-P
  • Visfatin Antibody (YA5011) Product Image IHC-P
  • Visfatin Antibody (YA5011) Product Image ICC
  • Visfatin Antibody (YA5011) Product Image ICC