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RPE65 Antibody (YA7182)

(Synonyms: LCA2; mRPE65; p63; rd12; Retinal pigment epithelium specific 61 kDa protein; RP20; RPE65; sRPE65)
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RPE65 Antibody (YA7182) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RPE65.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP, IF-Tissue

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • RPE65 Antibody (YA7182) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
Dilution Ratio 1:2000 1:10-1:50 1:1000 1:500

Product Details

Description

RPE65 Antibody (YA7182) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RPE65.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 61 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 61 kDa
Species Reactivity Database
Immunogen

Synthetic peptide within Human RPE65 aa 441-484 / 533.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    RPE65 (Retinal pigment epithelium 65 kDa protein) is a critical enzyme in the visual cycle that is specifically expressed in retinal pigment epithelial (RPE) cells. As a retinoid isomerohydrolase, RPE65 catalyzes the conversion of all-trans-retinyl esters to 11-cis-retinol, a vital process for visual phototransduction [1].RPE65 facilitates retinoid isomerization through its iron-binding site, maintaining the continuous supply of 11-cis-retinal in the retina - the essential precursor for rhodopsin regeneration [2]. Deficiencies in RPE65 function disrupt the visual cycle and are associated with various inherited retinal disorders, including Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa [3].

  • Subcellular Localization

    Lipid droplet; Endoplasmic reticulum; Nucleus

  • Expression


    Tissue_specificity:It is mainly expressed in adipose tissue, small intestine, heart, colon and stomach, with lower expression levels in brain, kidney and liver.

    Positive sample: Mouse eyeball tissue lysate, Rat eyeball tissue lysate, rat eyeball tissue, mouse eyeball tissue.

  • Isoforms & Post-Translational Modification

    Q96AQ7 has 4 isomers: Q96AQ7-1: 26754 Da (predicted); Q96AQ7-2: 18439 Da (predicted); Q96AQ7-3: 14013 Da (predicted); Q96AQ7-4: 27923 Da (predicted).
    Ubiquitinated and targeted to proteasomal degradation, resulting in a short half-life (about 15 minutes in 3T3-L1 cells). Protein stability depends on triaclyglycerol synthesis, fatty acid availability and lipid droplet formation

  • Subunit

    Homodimer (By similarity). Homooligomer; undergoes liquid-liquid phase separation (LLPS) via its N-terminus, facilitating lipid droplet fusion, occurs at the lipid droplet contact sites (By similarity). Interacts with CIDEA (PubMed:19843876). Interacts with PLIN1 (PubMed:23399566). Interacts with NFAT5; this interaction is direct and retains NFAT5 in the cytoplasm (By similarity). Interacts with CEBPB (By similarity). Interacts with isoform CLSTN3beta of CLSTN3; inhibiting the lipid transferase activity of CIDEC (By similarity)

  • SwissProt ID

    Q16518

  • Gene ID
    6121 [NCBI]
  • Synonyms

    LCA2; mRPE65; p63; rd12; Retinal pigment epithelium specific 61 kDa protein; RP20; RPE65; sRPE65

References

[1]. Gu SM, et al. Mutations in RPE65 cause autosomal recessive childhood-onset severe retinal dystrophy. Nat Genet. 1997 Oct;17(2):194-7. [Content Brief]

[2]. Moiseyev G, et al. RPE65 is the isomerohydrolase in the retinoid visual cycle. Proc Natl Acad Sci U S A. 2005 Aug 30;102(35):12413-8. [Content Brief]

[3]. Russell S, et al. Efficacy and safety of voretigene neparvovec (AAV2-hRPE65v2) in patients with RPE65-mediated inherited retinal dystrophy: a randomised, controlled, open-label, phase 3 trial. Lancet. 2017 Aug 26;390(10097):849-860. [Content Brief]

RPE65 Antibody (YA7182) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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