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  4. GALT Antibody (YA2925)

GALT Antibody (YA2925) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GALT.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay
  • Product Detail

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  • Descripciòn

Descripciòn

GALT Antibody (YA2925) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GALT.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Peso molecular
Predicted band size: 43 kDa;
Observed band size: 43 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthetic peptide of human GALT

Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IP
IP: Immunoprecipitation
1:20
Sensitivity Endogenous Pureza Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image
WB
  • Western blot analysis was performed on extracts from K562 (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Hela (lane 3, 15 μg), C6 (lane 4, 15 μg), Mouse liver (lane 5, 15 μg), and Rat brain (lane 6, 15 μg) using GALT Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:40000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Western blot analysis of extracts from Hela(lane 2(20ug) , C6(lane 3(20ug) and HEK293(lane 4(20ug) using GALT Antibody (HY-P83180) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function:Plays an important role in galactose metabolism
Isoforms & Post-Translational Modification:P07902 has 2 isomers: P07902-1: 43363 Da (predicted); P07902-2: 31107 Da (predicted).
Subunit:Homodimer
RRID
Database
Research Field

Signal Transduction

Synonyms
Gal-1-P uridylyltransferase; UDP-glucose--hexose-1-phosphate uridylyltransferase
Documentación
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

Nombre del producto:
GALT Antibody (YA2925)
Cat. No.:
HY-P83180
Cantidad:
MCE Japan Authorized Agent: