KDM5C/Jarid1C/SMCX Antibody (YA5159)

Cat. No.: HY-P85467: Data Sheet User Guide for Antibodies
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KDM5C/Jarid1C/SMCX Antibody (YA5159) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to KDM5C/Jarid1C/SMCX.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

KDM5C/Jarid1C/SMCX Antibody (YA5159) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to KDM5C/Jarid1C/SMCX.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 176 kDa;

Species Reactivity

Human, Mouse, Rat, Bovine, Dog

SwissProt ID

P41229

Gene ID

8242 [NCBI]

Immunogen

Purified recombinant human SmcX (C-terminus) protein fragments expressed in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:500

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code (PubMed:28262558). Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements. Represses the CLOCK-BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2 (By similarity)

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:It was expressed in all tissues tested. The highest levels were found in the brain and skeletal muscle.

Isoforms & Post-Translational Modification：P41229 has 5 isomers: P41229-1: 175720 Da (predicted); P41229-2: 175379 Da (predicted); P41229-3: 170561 Da (predicted); P41229-4: 155119 Da (predicted); P41229-5: 175592 Da (predicted).

Subunit：Part of two distinct complexes, one containing E2F6, and the other containing REST (PubMed:17468742). Interacts with ZMYND8 (PubMed:33323928)

Synonyms

KDM5C; DXS1272E; JARID1C; SMCX; XE169; Lysine-specific demethylase 5C; Histone demethylase JARID1C; Jumonji/ARID domain-containing protein 1C; Protein SmcX; Protein Xe169

Documentation

User Guide for Antibodies (1077 KB)