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Phospho-MEK1/2(Ser217/221) Antibody (YA5835)

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Based on 1 publication(s) in Google Scholar

Phospho-MEK1/2(Ser217/221) Antibody (YA5835) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-MEK1/2(Ser217/221).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG/Kappa

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Phospho-MEK1/2(Ser217/221) Antibody (YA5835) Product Image
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Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:5000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

Phospho-MEK1/2(Ser217/221) Antibody (YA5835) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-MEK1/2(Ser217/221).

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 44 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 44 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG/Kappa

RRID

AB_3719091

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
    Western blot analysis of extracts from C6(lane2(20μg), Hela(20μg), 3T3-L1(lane4(20μg) and HT-29(lane5(20μg) using Phospho-MEK1/2(Ser217/221) Antibody (YA5835)(HY-P86143). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
    Immunocytochemistry analysis of Hela cells labelingPhospho-MEK1/2(Ser217/221) With Phospho-MEK1/2(Ser217/221) antibody (HY-P86143) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Phospho-MEK1/2(Ser217/221) antibody (HY-P86143) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
    Immunocytochemistry analysis of Hela cells labeling Phospho-MEK1/2(Ser217/221) With Phospho-MEK1/2(Ser217/221) antibody (HY-P86143) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Phospho-MEK1/2(Ser217/221) antibody (HY-P86143) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    MEK1/2 is a P36507: Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases. Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation
    Q02750: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis
    [1].

  • Subcellular Localization

    P36507: Cytoplasm; Membrane; Peripheral membrane protein
    Q02750: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body; Cytoplasm; Nucleus; Membrane; Peripheral membrane protein

  • Expression


    Tissue_specificity: Q02750: Widely expressed, with extremely low levels in brain

  • Isoforms & Post-Translational Modification

    Q02750 has 2 isomers: Q02750-1: 43439 Da (predicted); Q02750-2: 40764 Da (predicted).
    Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (BRAF or MEKK1) positively regulates kinase activity (PubMed:29433126, PubMed:8131746). Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK (PubMed:16129686). MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK (PubMed:16129686). Autophosphorylated at Ser-218 and Ser-222, autophosphosphorylation is promoted by NEK10 following UV irradiation (PubMed:20956560);(Microbial infection) Acetylation by Yersinia YopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway

  • Subunit

    P36507: Interacts with MORG1 (By similarity). Interacts with SGK1 (PubMed:19447520). Interacts with KSR1 (PubMed:10409742). Interacts with KSR1 and BRAF; the interaction with KSR1 mediates KSR1-BRAF dimerization (PubMed:29433126). Interacts with GLS (PubMed:22538822)
    Q02750: Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3/ERK1 and RGS14 (By similarity). Forms a heterodimer with MAP2K2/MEK2 (By similarity). Forms heterodimers with KSR2 which further dimerize to form tetramers (By similarity).

  • SwissProt ID

    P36507 / Q02750

  • Gene ID
    5604 [NCBI] / 5605 [NCBI]

Phospho-MEK1/2(Ser217/221) Antibody (YA5835) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from C6(lane2(20μg), Hela(20μg), 3T3-L1(lane4(20μg) and HT-29(lane5(20μg) using Phospho-MEK1/2(Ser217/221) Antibody (YA5835)(HY-P86143). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labelingPhospho-MEK1/2(Ser217/221) With Phospho-MEK1/2(Ser217/221) antibody (HY-P86143) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Phospho-MEK1/2(Ser217/221) antibody (HY-P86143) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Phospho-MEK1/2(Ser217/221) With Phospho-MEK1/2(Ser217/221) antibody (HY-P86143) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Phospho-MEK1/2(Ser217/221) antibody (HY-P86143) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-MEK1/2(Ser217/221) Antibody (YA5835) Product Image
    1/16
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Phospho-MEK1/2(Ser217/221) Antibody (YA5835) Product Image
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from C6(lane2(20μg), Hela(20μg), 3T3-L1(lane4(20μg) and HT-29(lane5(20μg) using Phospho-MEK1/2(Ser217/221) Antibody (YA5835)(HY-P86143). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labelingPhospho-MEK1/2(Ser217/221) With Phospho-MEK1/2(Ser217/221) antibody (HY-P86143) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Phospho-MEK1/2(Ser217/221) antibody (HY-P86143) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Phospho-MEK1/2(Ser217/221) With Phospho-MEK1/2(Ser217/221) antibody (HY-P86143) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Phospho-MEK1/2(Ser217/221) antibody (HY-P86143) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Experimental Validation Results for Phospho-MEK1/2(Ser217/221) Antibody (YA5835)
  • Phospho-MEK1/2(Ser217/221) Antibody (YA5835) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C6(lane2(20μg), Hela(20μg), 3T3-L1(lane4(20μg) and HT-29(lane5(20μg) using Phospho-MEK1/2(Ser217/221) Antibody (YA5835)(HY-P86143). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Phospho-MEK1/2(Ser217/221) Antibody (YA5835) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Phospho-MEK1/2(Ser217/221) Antibody (YA5835) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Phospho-MEK1/2(Ser217/221) Antibody (YA5835) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Phospho-MEK1/2(Ser217/221) Antibody (YA5835) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Phospho-MEK1/2(Ser217/221) Antibody (YA5835) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Phospho-MEK1/2(Ser217/221) Antibody (YA5835) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using Phospho-MEK1/2(Ser217/221) Antibody (YA5835). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86143, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Phospho-MEK1/2(Ser217/221) Antibody (YA5835) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labelingPhospho-MEK1/2(Ser217/221) With Phospho-MEK1/2(Ser217/221) antibody (HY-P86143) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Phospho-MEK1/2(Ser217/221) antibody (HY-P86143) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-MEK1/2(Ser217/221) Antibody (YA5835) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Phospho-MEK1/2(Ser217/221) With Phospho-MEK1/2(Ser217/221) antibody (HY-P86143) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Phospho-MEK1/2(Ser217/221) antibody (HY-P86143) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-MEK1/2(Ser217/221) Antibody (YA5835) Product Image WB
  • Phospho-MEK1/2(Ser217/221) Antibody (YA5835) Product Image IHC-P
  • Phospho-MEK1/2(Ser217/221) Antibody (YA5835) Product Image IHC-P
  • Phospho-MEK1/2(Ser217/221) Antibody (YA5835) Product Image IHC-P
  • Phospho-MEK1/2(Ser217/221) Antibody (YA5835) Product Image IHC-P
  • Phospho-MEK1/2(Ser217/221) Antibody (YA5835) Product Image IHC-P
  • Phospho-MEK1/2(Ser217/221) Antibody (YA5835) Product Image IHC-P
  • Phospho-MEK1/2(Ser217/221) Antibody (YA5835) Product Image ICC
  • Phospho-MEK1/2(Ser217/221) Antibody (YA5835) Product Image ICC