Fyn Antibody (YA7509)
(Synonyms: Tyrosine-protein kinase Fyn, Proto-oncogene Syn, Proto-oncogene c-Fyn, Src-like kinase, p59-Fyn, SLK, FYN)Fyn Antibody (YA7509) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Fyn.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:2000-1:10000 | 1:200-1:1000 | 1:5000-1:20000 |
Product Details
Fyn Antibody (YA7509) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Fyn.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 61 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 61 kDa
The exact sequence is proprietary to MCE.
Endogenous
Protein A affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Fyn is a Src family tyrosine kinase represented in brain by the neuronal isoform p59FynB, where Src and Fyn constitute the two main tyrosine kinases involved in neuronal growth and myelination[1]. Mechanistically, Fyn participates in Src-family signaling modules that regulate inflammation, apoptosis, neuroinflammation, and epileptogenesis in experimental systems[2][3]. In pulmonary endothelial inflammation models, fyn knockdown increased caspase-3 activity, reduced viable cell numbers, and intensified LPS-induced lung injury in fyn−/− mice, identifying Fyn as a negative regulator of inflammation-induced apoptosis[2]. In temporal lobe epilepsy models, the Fyn-PKCδ pathway linked status epilepticus to neuroinflammation and epileptogenesis, while saracatinib reduced seizures, gliosis, neurodegeneration, phosphorylated Fyn/Src-419, PKCδ-tyr311, nitrooxidative stress, and proinflammatory cytokines[3]. Compared with related isoforms, Fyn showed an opposite endothelial apoptosis phenotype to Yes, because yes knockdown lowered caspase-3 activity and increased viable cells under cytomix stimulation[2]. For experimental applications, saracatinib functions as a Src/Fyn tyrosine kinase inhibitor for probing epileptogenesis-associated neuroinflammatory and nitrooxidative pathways[3].
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Subcellular Localization
Cytoplasm,Nucleus,Cell membrane,Perikaryon
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Expression
Tissue_Specificity: Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes -
Isoforms & Post-Translational Modification
P06241 has three isomers: P06241-1: 60762 Da (predicted); P06241-2: 60141 Da (predicted); P06241-3: 54513 Da (predicted).
Autophosphorylated at Tyr-420 (By similarity)丨Palmitoylated -
Subunit
Interacts (via its SH3 domain) with PIK3R1 and PRMT8
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SwissProt ID
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Synonyms
Tyrosine-protein kinase Fyn, Proto-oncogene Syn, Proto-oncogene c-Fyn, Src-like kinase, p59-Fyn, SLK, FYN
Documentation
References
[1]. Rouer E. Les isoformes neuronales des tyrosines kinases Src, et al. Les isoformes neuronales des tyrosines kinases Src, Fyn et Lck : rôle particulier de la p56lckN dans la survie neuronale [Neuronal isoforms of Src, Fyn and Lck tyrosine kinases: A specific role for p56lckN in neuron protection]. C R Biol. 2010 Jan;333(1):1-10. French. [Content Brief]
[3]. Sharma S, et al. Mechanisms of disease-modifying effect of saracatinib (AZD0530), a Src/Fyn tyrosine kinase inhibitor, in the rat kainate model of temporal lobe epilepsy. Neurobiol Dis. 2021 Aug;156:105410. [Content Brief]
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