Annexin A1/ANXA1 Antibody (YA832)(PBS only)

Cat. No.: HY-P80544A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

Annexin A1/ANXA1 Antibody (YA832) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Annexin A1/ANXA1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Annexin A1/ANXA1 Antibody (YA832) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Annexin A1/ANXA1.

Host

Mouse

Molecular Weight

Predicted band size: 39 kDa;

Observed band size: 39 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Monkey

SwissProt ID

P04083

Gene ID

301 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Annexin A1 beta.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Purity	affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed:8425544). Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed:17008549). Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed:17008549). Has no effect on unstimulated T cells (PubMed:17008549). Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (PubMed:19625660). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678). Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (By similarity); Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades (PubMed:15187149, PubMed:22879591, PubMed:25664854). Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (PubMed:15187149). Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration (PubMed:15187149). Promotes resolution of inflammation and wound healing (PubMed:25664854). Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2 (PubMed:22879591)

Subcellular Localization：Nucleus; Cytoplasm; Cell projection, cilium; Cell membrane; Membrane; Peripheral membrane protein; Endosome membrane; Peripheral membrane protein; Basolateral cell membrane; Apical cell membrane; Lateral cell membrane; Secreted; Secreted, extracellular space; Cell membrane; Peripheral membrane protein; Extracellular side; Secreted, extracellular exosome; Cytoplasmic vesicle, secretory vesicle lumen; Cell projection, phagocytic cup; Early endosome; Cytoplasmic vesicle membrane; Peripheral membrane protein

Expression：
Tissue_specificity:Detected in resting neutrophils (PubMed: 10772777) . Detected in peripheral blood T cells (PubMed: 17008549) . Detected in extracellular vesicles of inflammatory bowel disease serum, but not detected in serum of healthy donors (PubMed: 25664854) . Detected in placenta (protein level) (PubMed: 2532504) . Detected in liver.

Subunit：Homodimer; non-covalently linked (By similarity). Homodimer; linked by transglutamylation (PubMed:2532504).

Research Field

Signal Transduction

Synonyms

ANXA1; ANX1; LPC1; Annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35

Documentation

User Guide for Antibodies (1077 KB)