KBTBD8 Antibody (YA4375)

Cat. No.: HY-P84678: Data Sheet COA
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KBTBD8 Antibody (YA4375) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to KBTBD8.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

KBTBD8 Antibody (YA4375) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to KBTBD8.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 69 kDa;

Observed band size: 69 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q8NFY9

Gene ID

84541 [NCBI]

Immunogen

Purified recombinant fragment of human KBTBD8 (AA: 264-464) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB FC

Western blot analysis of extracts from A431 (lane 2(20μg), Jurkat (lane 3(20μg), HeLa (lane 4(20μg) and K562 (lane 5(20μg) using KBTBD8 Antibody (HY-P84678) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from HEK293(lane 1(40μg) ),Hela(lane 2(40μg) ),HepG2(lane 3(40μg) ),Jurkat(lane 4(40μg) ), K562(lane 5(40μg) ),NIH/3T3(lane 6(40μg) )and A431(lane 7(40μg) ) using KBTBD8 antibody(30768P). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，20035, 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
Western blot analysis of extracts from MOLT4(lane 1(40μg) ),Raji(lane 2(40μg) ),Raw264.7(lane 3(40μg) ),F9(lane 4(40μg) ), COS-7(lane 5(40μg) ),PC-12(lane 6(40μg) ),NIH/3T3(lane 7(40μg) ),Jurkat(lane 8(40μg) ),A431(lane 9(40μg) ),MCF-7(lane 10(40μg) ))and PANC-1(lane 11(40μg) ) using KBTBD8 antibody(30768P). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，20035, 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
Flow cytometric analysis of 1X10^6 A431 cells labeling KBTBD8 Antibody(30768P,green). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, gray) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (red).

Background

Function：Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of neural crest specification (PubMed:26399832). The BCR(KBTBD8) complex acts by mediating monoubiquitination of NOLC1 and TCOF1: monoubiquitination promotes the formation of a NOLC1-TCOF1 complex that acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832)

Subcellular Localization：Cytoplasm, cytoskeleton, spindle; Golgi apparatus

Isoforms & Post-Translational Modification：Q8NFY9 has 2 isomers: Q8NFY9-1: 68823 Da (predicted); Q8NFY9-2: 18827 Da (predicted).

Subunit：Component of the BCR(KBTBD8) E3 ubiquitin ligase complex, at least composed of CUL3, KBTBD8 and RBX1 (PubMed:26399832)

RRID

AB_3718963

Synonyms

TA-KRP

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)