EphB6 Antibody (YA3524)(PBS only)

Cat. No.: HY-P83827A: Data Sheet User Guide for Antibodies
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EphB6 Antibody (YA3524) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EphB6.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

EphB6 Antibody (YA3524) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EphB6.

Host

Mouse

Molecular Weight

Predicted band size: 111 kDa;

Observed band size: 120 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O15197

Gene ID

2051 [NCBI]

Immunogen

Purified recombinant fragment of EphB6 (DEVSVLDDQRRLTRTFEAC).

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2

Subcellular Localization：Membrane; Single-pass type I membrane protein; Secreted

Expression：
Tissue_specificity:Expressed in brain tissue. Expressed in non-invasive breast cancer cell lines (protein level) . Strongly expressed in the brain and pancreas, and weakly expressed in other tissues such as the heart, placenta, lung, liver, skeletal muscle, and kidney. Expressed in non-invasive breast tumors, but not in metastatic lesions. Isomer 3 is expressed in cell lines of glioblastoma, anaplastic astrocytoma, gliosarcoma, and astrocytoma. Isomer 3 was not detected in normal tissues.

Isoforms & Post-Translational Modification：O15197 has 3 isomers: O15197-1: 110700 Da (predicted); O15197-2: 81211 Da (predicted); O15197-3: 57498 Da (predicted).
Ligand-binding increases phosphorylation on tyrosine residues. Phosphorylation on tyrosine residues is mediated by transphosphorylation by the catalytically active EPHB1 in a ligand-independent manner. Tyrosine phosphorylation of the receptor may act as a switch on the functional transition from cell adhesion/attraction to de-adhesion/repulsion

Subunit：Interacts with CBL and EPHB1. Interacts with FYN; this interaction takes place in a ligand-independent manner

Synonyms

HEP; MGC129910; MGC129911

Documentation

User Guide for Antibodies (1077 KB)