CD14 Antibody (YA6144)
(Synonyms: Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD antigen CD14; [Cleaved into: Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form])CD14 Antibody (YA6144) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD14.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, IHC-P, ICC/IF, IP, ELISA
-
Reactivity :
Human, Mouse
-
Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
|
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
|
IP
IP: Immunoprecipitation
|
|---|---|---|---|---|---|
| Dilution Ratio | 1:400-1:1000 | 1:1000-1:5000 | 1:200-1:1000 | 1:5000-1:20000 | 1:50-1:200 |
Product Details
CD14 Antibody (YA6144) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD14.
-
Host Rabbit
-
Clonality Monoclonal
-
Species ReactivityHuman, Mouse
-
Observed Molecular WeightObserved band size: 52 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 39 kDa
Protein A
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
CD14 is an important innate immune pattern recognition receptor, primarily serving as a component of the lipopolysaccharide (LPS) receptor complex. It plays a crucial role in recognizing Gram-negative bacteria and their products, and in activating inflammatory responses. CD14 itself lacks a transmembrane signal transduction structure; therefore, it typically functions as a co-receptor in synergy with TLR4 and MD-2.
-
Subcellular Localization
Cell membrane; Lipid-anchor, GPI-anchor; Secreted; Membrane raft; Golgi apparatus
-
Expression
Tissue_specificity:Protein levels were detected on macrophages (PubMed: 1698311) . It is strongly expressed on the surface of monocytes and weakly expressed on the surface of granulocytes; it is also expressed in macrophages of most tissues.
Induction:The expression in monocytes is highly induced by 27-hydroxycholesterol, priming monocytes/macrophages such that LPS-mediated inflammatory reaction is accelerated. Secretion of soluble CD14 is also enhanced -
Subunit
Interacts with LPS-bound LPB. Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4.
-
SwissProt ID
-
Synonyms
Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD antigen CD14; [Cleaved into: Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form]
Documentation
[1]. Gangloff SC, et al. Influence of CD14 on ligand interactions between lipopolysaccharide and its receptor complex. J Immunol. 2005 Sep 15;175(6):3940-5. [Content Brief]
[2]. Jiang Z, et al. CD14 is required for MyD88-independent LPS signaling. Nat Immunol. 2005 Jun;6(6):565-70. [Content Brief]
[3]. Haziot A, et al. Resistance to endotoxin shock and reduced dissemination of gram-negative bacteria in CD14-deficient mice. Immunity. 1996 Apr;4(4):407-14. [Content Brief]
[4]. Drage MG, et al. TLR2 and its co-receptors determine responses of macrophages and dendritic cells to lipoproteins of Mycobacterium tuberculosis. Cell Immunol. 2009;258(1):29-37. [Content Brief]
Powered by Bioz
