PASK Antibody (YA8235)(PBS only)

Cat. No.: HY-P88551A: Data Sheet User Guide for Antibodies
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PASK Antibody (YA8235) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to PASK.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PASK Antibody (YA8235) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to PASK.

Host

Mouse

Molecular Weight

Predicted band size: 142.7 kDa

Species Reactivity

Human

SwissProt ID

Q96RG2

Immunogen

Full length human recombinant protein of human PASK produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate in respiratory regulation

Subcellular Localization：Cytoplasm,Nucleus

Expression：
Tissue_Specificity: Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta. Present in germ cells of testis and in the midpiece of sperm tails (at protein level)

Isoforms & Post-Translational Modification：Q96RG2 has three isomers: Q96RG2-1: 142929 Da (predicted); Q96RG2-2: 143684 Da (predicted); Q96RG2-4: 123048 Da (predicted).
Autophosphorylated on Thr-1161 and Thr-1165

Synonyms

PASKIN; STK37

Documentation

User Guide for Antibodies (1077 KB)