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IRAK-1 Antibody (YA2454)

(Synonyms: Il1rak; IRAK; Irak1; IRAK1-S; mPLK; Pelle; Pelle homolog; Pelle-like protein kinase; Plpk)
Customer Review

Based on 1 Customer Validation

IRAK-1 Antibody (YA2454) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IRAK-1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for IRAK-1 Antibody (YA2454)
  • Experimental Validation Results for IRAK-1 Antibody (YA2454)
  • Experimental Validation Results for IRAK-1 Antibody (YA2454)
  • IRAK-1 Antibody (YA2454) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50

Product Details

Description

IRAK-1 Antibody (YA2454) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IRAK-1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 77 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 77 kDa
Species Reactivity Database

Entrez Gene: 3654 Human

SwissProt: P51617 Human

Immunogen

A synthesized peptide derived from human IRAK aa200-500.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104808

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IRAK-1 Antibody (YA2454)
    Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Monoamine IRAK1 Antibody (HY-P82709) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IRAK-1 Antibody (YA2454)
    Immunocytochemistry analysis of Hela cells labeling IRAK1 Antibody (HY-P82709) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with IRAK1 Antibody (HY-P82709) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for IRAK-1 Antibody (YA2454)
    Immunocytochemistry analysis of Hela cells labeling IRAK1 Antibody (HY-P82709) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with IRAK1 Antibody (HY-P82709) at 1/150 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Interleukin-1 receptor-associated kinase 1 (IRAK1) is a serine-threonine kinase that mediates signaling downstream of Toll-like receptors (TLRs) and interleukin-1 receptor (IL-1R) complexes, promoting innate immune and inflammatory responses[1][2]. Mechanistically, IRAK1 interacts with the adaptor protein MyD88 and is recruited to the myddosome, where it undergoes phosphorylation and contributes to the activation of NF-κB and MAPK pathways[2][3]. Compared with its paralog IRAK4, IRAK1 exhibits distinct roles in hematologic malignancies, being preferentially expressed and required for maintaining viability and progenitor function in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML) [4][5]. IRAK1 also regulates cancer stemness and chemoresistance in hepatocellular carcinoma through the IRAK1/IRAK4/AP-1/AKR1B10 signaling cascade, influencing self-renewal and tumorigenicity[6]. Alternative splicing generates the IRAK1b isoform, which is kinase-inactive but stable, adding regulatory complexity to IL-1 signaling[7]. Pharmacologically, selective IRAK1 inhibitors, including dual IRAK1/4 compounds and covalent degraders, effectively suppress inflammatory responses, enhance chemotherapy sensitivity, and reduce leukemic or tumor-initiating cell function in preclinical models[8][9][10][5][11][12]. These findings underscore the importance of IRAK1 in innate immunity, oncogenesis, and as a target for therapeutic intervention, while differentiating its function and regulation from other IRAK family members[13][4][5].

  • Subcellular Localization

    Cytoplasm; Nucleus; Lipid droplet

  • Expression


    Tissue_specificity:In all tissues tested, both isomer 1 and isomer 2 were widely expressed, with isomer 1 being expressed at a higher intensity than isomer 2.

  • Isoforms & Post-Translational Modification

    P51617 has 4 isomers: P51617-1: 76537 Da (predicted); P51617-2: 73421 Da (predicted); P51617-3: 74560 Da (predicted); P51617-4: 68022 Da (predicted).
    Following recruitment on the activated receptor complex, phosphorylated on Thr-209, probably by IRAK4, resulting in a conformational change of the kinase domain, allowing further phosphorylations to take place. Thr-387 phosphorylation in the activation loop is required to achieve full enzymatic activity;Polyubiquitinated by TRAF6 after cell stimulation with IL-1-beta by PELI1, PELI2 and PELI3. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. Ubiquitination promotes interaction with NEMO/IKBKG. Also sumoylated; leading to nuclear translocation

  • Subunit

    Forms a complex with TRAF6, PELI1, IRAK4 and MYD88 (PubMed:16951688). Direct binding of SMAD6 to PELI1 prevents complex formation and hence negatively regulates IL1R-TLR signaling and eventually NF-kappa-B-mediated gene expression (PubMed:16951688).

  • SwissProt ID

    P51617

  • Gene ID
    3654 [NCBI]
  • Synonyms

    Il1rak; IRAK; Irak1; IRAK1-S; mPLK; Pelle; Pelle homolog; Pelle-like protein kinase; Plpk

  • Research Field

    Immunology

References

[1]. Singer JW, et al. Inhibition of interleukin-1 receptor-associated kinase 1 (IRAK1) as a therapeutic strategy. Oncotarget. 2018 Sep 7;9(70):33416-33439. [Content Brief]

[2]. Ordureau A, et al. The IRAK-catalysed activation of the E3 ligase function of Pellino isoforms induces the Lys63-linked polyubiquitination of IRAK1. Biochem J. 2008 Jan 1;409(1):43-52. [Content Brief]

[3]. Hou Y, et al. IRAK Inhibitor Protects the Intestinal Tract of Necrotizing Enterocolitis by Inhibiting the Toll-Like Receptor (TLR) Inflammatory Signaling Pathway in Rats. Med Sci Monit. 2018 May 22;24:3366-3373. [Content Brief]

[4]. Hermans A, et al. A 3D-Printed and Freely Available Device to Measure the Zebrafish Optokinetic Response Before and After Injury. Zebrafish. 2024 Apr;21(2):144-148. [Content Brief]

[5]. Gosu V, et al. Molecular evolution and structural features of IRAK family members. PLoS One. 2012;7(11):e49771. [Content Brief]

[6]. Rhyasen GW, et al. Differential IRAK signaling in hematologic malignancies. Exp Hematol. 2013 Dec;41(12):1005-7. [Content Brief]

[7]. Cheng BY, et al. IRAK1 Augments Cancer Stemness and Drug Resistance via the AP-1/AKR1B10 Signaling Cascade in Hepatocellular Carcinoma. Cancer Res. 2018 May 1;78(9):2332-2342. [Content Brief]

[8]. Jensen LE, et al. IRAK1b, a novel alternative splice variant of interleukin-1 receptor-associated kinase (IRAK), mediates interleukin-1 signaling and has prolonged stability. J Biol Chem. 2001 Aug 3;276(31):29037-44. [Content Brief]

[9]. Kim KM, et al. Recent Advances in IRAK1: Pharmacological and Therapeutic Aspects. Molecules. 2024 May 9;29(10):2226. [Content Brief]

[10]. Sutter PJ, et al. Optimization of IRAK1/4/pan-FLT3 kinase inhibitors as treatments for acute myeloid leukemia. Bioorg Med Chem Lett. 2025 Dec 15;129:130355. [Content Brief]

[11]. Bennett J, et al. Paralog-specific signaling by IRAK1/4 maintains MyD88-independent functions in MDS/AML. Blood. 2023 Sep 14;142(11):989-1007. [Content Brief]

[12]. Yan B, et al. Inhibition of IRAK 1/4 alleviates colitis by inhibiting TLR4/ NF-κB pathway and protecting the intestinal barrier. Bosn J Basic Med Sci. 2022 Oct 23;22(6):872-881. [Content Brief]

[13]. Braselmann S, et al. AB0058 CELL-TYPE SPECIFIC REGULATION OF IL-1R SIGNALING BY R835, A DUAL IRAK1/4 INHIBITOR[J]. Annals of the Rheumatic Diseases, 2020, 79: 1331.

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IRAK-1 Antibody (YA2454) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Monoamine IRAK1 Antibody (HY-P82709) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling IRAK1 Antibody (HY-P82709) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with IRAK1 Antibody (HY-P82709) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling IRAK1 Antibody (HY-P82709) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with IRAK1 Antibody (HY-P82709) at 1/150 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • IRAK-1 Antibody (YA2454) Product Image
    1/16
  • Experimental Validation Results for IRAK-1 Antibody (YA2454)
  • Experimental Validation Results for IRAK-1 Antibody (YA2454)
  • Experimental Validation Results for IRAK-1 Antibody (YA2454)
  • IRAK-1 Antibody (YA2454) Product Image
  • Experimental Validation Results for IRAK-1 Antibody (YA2454)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Monoamine IRAK1 Antibody (HY-P82709) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IRAK-1 Antibody (YA2454)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling IRAK1 Antibody (HY-P82709) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with IRAK1 Antibody (HY-P82709) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for IRAK-1 Antibody (YA2454)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling IRAK1 Antibody (HY-P82709) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with IRAK1 Antibody (HY-P82709) at 1/150 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for IRAK-1 Antibody (YA2454)
  • Experimental Validation Results for IRAK-1 Antibody (YA2454)
  • Experimental Validation Results for IRAK-1 Antibody (YA2454)
  • IRAK-1 Antibody (YA2454) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Monoamine IRAK1 Antibody (HY-P82709) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • IRAK-1 Antibody (YA2454) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling IRAK1 Antibody (HY-P82709) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with IRAK1 Antibody (HY-P82709) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • IRAK-1 Antibody (YA2454) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling IRAK1 Antibody (HY-P82709) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with IRAK1 Antibody (HY-P82709) at 1/150 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • IRAK-1 Antibody (YA2454) Product Image WB
  • IRAK-1 Antibody (YA2454) Product Image ICC
  • IRAK-1 Antibody (YA2454) Product Image ICC