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PHD3 Antibody (YA1612)

(Synonyms: PHD3; HIFPH3; HIFP4H3)
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Based on 1 Customer Validation

PHD3 Antibody (YA1612) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHD3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PHD3 Antibody (YA1612)
  • PHD3 Antibody (YA1612) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:20

Product Details

Description

PHD3 Antibody (YA1612) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHD3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 27 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 27 kDa
Species Reactivity Database
Immunogen

Recombinant protein of human PHD3 aa1-239.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103965

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PHD3 Antibody (YA1612)
    Western blot analysis of extracts from A549 (lane 2(20μg)),Raw264.7 (lane 3(20μg)),PC-12 (lane 4(20μg)) ,3T3 (lane 5(20μg)) and Rat kidney (lane 6(20μg)) using PHD3 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    PHD3 is a HIF prolyl hydroxylase that regulates HIF-1 protein levels in mammalian cells. HIF-1α prolyl hydroxylases mainly include PHD1, PHD2, and PHD3. These three enzymes have sequence-similar catalytic domains and conserve amino acid residues for coordinating Fe (II) ions. Under hypoxic conditions, due to insufficient oxygen supply, the hydroxylase activity of PHD3 decreases, leading to HIF-1α accumulation and inducing the expression of HRE-regulated target genes. Therefore, PHD is considered an intracellular oxygen sensor. PHD3 is expressed at the highest level in the heart.

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Expression


    Tissue_specificity:Widely expressed at low levels. Expressed at higher levels in human heart (cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle) , lung and placenta, and in fetal spleen, heart and skeletal muscle. Also expressed in pancreas. Localized to pancreatic acini and islet cells

    Induction:Induced by hypoxia in a number of cells including neutrophils and certain cancer cell lines. Up-regulated 10-fold in pancreatic cancers

  • Subunit

    Interacts with BCL2 (via its BH4 domain); the interaction disrupts the BAX-BCL4 complex inhibiting the anti-apoptotic activity of BCL2 (PubMed:20849813). Interacts with WDR83; the interaction leads to almost complete elimination of HIF-mediated reporter activity (By similarity). Interacts with ADRB2; the interaction hydroxylates ADRB2 facilitating its ubiquitination by the VHL-E3 ligase complex (PubMed:19584355). Interacts with PAX2; the interaction targets PAX2 for destruction (PubMed:21575608). Interacts with PKM; the interaction hydroxylates PKM in hypoxia (PubMed:21483450, PubMed:21620138). Interacts with LIMD1, WTIP and AJUBA (PubMed:22286099)

  • SwissProt ID

    Q9H6Z9

  • Gene ID
    112399 [NCBI]
  • Synonyms

    PHD3; HIFPH3; HIFP4H3

  • Research Field

    Cardiovascular

[1]. Yasumoto K, et al. Role of the intracellular localization of HIF-prolyl hydroxylases. Biochim Biophys Acta. 2009 May;1793(5):792-7. [Content Brief]

PHD3 Antibody (YA1612) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from A549 (lane 2(20μg)),Raw264.7 (lane 3(20μg)),PC-12 (lane 4(20μg)) ,3T3 (lane 5(20μg)) and Rat kidney (lane 6(20μg)) using PHD3 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PHD3 Antibody (YA1612) Product Image
    1/16
  • Experimental Validation Results for PHD3 Antibody (YA1612)
  • PHD3 Antibody (YA1612) Product Image
  • Experimental Validation Results for PHD3 Antibody (YA1612)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from A549 (lane 2(20μg)),Raw264.7 (lane 3(20μg)),PC-12 (lane 4(20μg)) ,3T3 (lane 5(20μg)) and Rat kidney (lane 6(20μg)) using PHD3 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PHD3 Antibody (YA1612)
  • PHD3 Antibody (YA1612) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from A549 (lane 2(20μg)),Raw264.7 (lane 3(20μg)),PC-12 (lane 4(20μg)) ,3T3 (lane 5(20μg)) and Rat kidney (lane 6(20μg)) using PHD3 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PHD3 Antibody (YA1612) Product Image WB