Thioredoxin Antibody (YA4612)(PBS only)
(Synonyms: Thioredoxin)Thioredoxin Antibody (YA4612) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Thioredoxin.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, ELISA
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Reactivity :
Tag
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:100-1:1000 | 1:100-1:200 | 1:200-1:400 | 1:10000 |
Product Details
Thioredoxin Antibody (YA4612) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Thioredoxin.
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Host Mouse
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Species ReactivityTag
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Observed Molecular WeightObserved band size: 12 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 12 kDa
Purified recombinant fusion protein with Thioredoxin (TRX) aa 1-101.
affinity purified.
Non-conjugated
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity; ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55)[1][2][3][4][5][6][7].
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Subcellular Localization
Nucleus; Cytoplasm; Secreted
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Expression
Induction:Up-regulated by ionizing radiation -
Isoforms & Post-Translational Modification
P10599 has 2 isomers: P10599-1: 11737 Da (predicted); P10599-2: 9452 Da (predicted).
In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins;In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation -
Subunit
Homodimer; disulfide-linked (PubMed:17260951, PubMed:9369469). Interacts with TXNIP through the redox-active site (PubMed:17260951). Interacts with MAP3K5 and CASP3 (PubMed:15246877). In case of infection, interacts with S.typhimurium protein slrP (PubMed:19690162). Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA-binding activity in a redox-dependent manner (PubMed:9108029)
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SwissProt ID
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Synonyms
Thioredoxin
Documentation
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