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  5. MUM1 Antibody (YA2408)

MUM1 Antibody (YA2408) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MUM1.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

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  • Beschreibung

Beschreibung

MUM1 Antibody (YA2408) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MUM1.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Molekulargewicht
Predicted band size: 52 kDa;
Observed band size: 52 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human MUM1 aa144-450/451.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
IP
IP: Immunoprecipitation
1:100
FC
FC: Flow Cytometry
1:50-1:100
Sensitivity Endogenous Reinheit Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Versand

Shipping with blue ice.
Verification Image
ALL WB ICC

  • Western blot analysis of extracts from Ramos(lane 2(20ug) and Ramos(lane 3(40ug) using MUM1 Antibody (HY-P82663) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of Ramos cells labeling Interferon Regulatory Factor 4 Antibody with Interferon Regulatory Factor 4 Antibody Antibody(HY-P82663) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Interferon Regulatory Factor 4 Antibody Antibody(HY-P82663) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of Ramos cells labeling Interferon Regulatory Factor 4 Antibody with Interferon Regulatory Factor 4 Antibody Antibody(HY-P82663) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Interferon Regulatory Factor 4 Antibody Antibody(HY-P82663) at 1/100 dilution in BSA for Immunol Staining at 4 ℃Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background
Function：Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes
Subcellular Localization：Nucleus; Cytoplasm
Expression：
Tissue_specificity:lymphocytes

Induction:Not induced by interferons
Isoforms & Post-Translational Modification：Q15306 has 2 isomers: Q15306-1: 51772 Da (predicted); Q15306-2: 51644 Da (predicted).
Phosphorylation by ROCK2 regulates IL-17 and IL-21 production
Subunit：Interacts with SPIB (PubMed:10196196). Interacts with DEF6 (PubMed:12651066). Interacts with SPI1 (PubMed:33951726)
RRID
Database

Entrez Gene: 3662 Human

SwissProt: Q15306 Human

OMIM: 254500 Human

Research Field

Tags & Cell Markers
Synonyms
IRF4; MUM1; Interferon regulatory factor 4; IRF-4; Lymphocyte-specific interferon regulatory factor; LSIRF; Multiple myeloma oncogene 1; NF-EM5
Dokumentation
SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

MUM1 Antibody (YA2408) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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