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Beclin 1 Antibody (YA6368)

(Synonyms: Beclin-1; ATG6; beclin1; VPS30; Beclin 1 like protein 1; 4921513J16Rik; 5430417M23Rik; ATG6; BECLIN 1; BECN1; MGC6843; BECN1_HUMAN.)
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Based on 1 Customer Validation

Beclin 1 Antibody (YA6368) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Beclin 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    IHC-P, WB, IF-Tissue, IHC-F

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Beclin 1 Antibody (YA6368)
  • Beclin 1 Antibody (YA6368) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
Dilution Ratio 1:50-200 1:100-1000 1:50-200 1:50-200

Product Details

Description

Beclin 1 Antibody (YA6368) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Beclin 1.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 50 kDa
Immunogen

Recombinant human Beclin 1 protein aa 141-450/450

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719203

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Beclin 1 Antibody (YA6368)
    Western blot analysis of extracts from HeLa(lane 2(20μg) , HepG2(lane 3(20μg) ,NIH-3T3(lane 4(20μg)and C6(lane 5(20μg) using Beclin 1 Antibody（HY-P86676）.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Beclin 1, also named Atg6, is highly conserved in eukaryotes and belongs to theautophagy-related (Atg) family of proteins. Beclin 1 is a well-established regulator of autophagy, a process essential for mammalian survival. Beclin 1 performs both of its autophagy and membrane trafficking functions by interacting with several other proteins—primarily vacuolar protein sorting-associated protein 15 (VPS15), VPS34, UV radiation resistance-associated gene product (UVRAG), and autophagy-related protein 14 (ATG14) . Together, these assemble into two different Class III PI3K complexes, Complex 1 (C1) and Complex 2 (C2), depending on whether ATG14 or UVRAG respectively is present. Within these complexes, the catalytic lipid kinase subunit, VSP34, is responsible for the phosphorylation of PtdIns, which then mediates autophagy and/or membrane trafficking functions through the recruitment of effector proteins.

  • Subcellular Localization

    Cytoplasm; Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein; Endosome membrane; Peripheral membrane protein; Endoplasmic reticulum membrane; Peripheral membrane protein; Mitochondrion membrane; Peripheral membrane protein; Endosome; Cytoplasmic vesicle, autophagosome; Mitochondrion; Nucleus; Cytoplasm; Mitochondrion

  • Expression


    Tissue_specificity:general expression

  • Subunit

    A homodimeric form is proposed to exist; this metastable form readily transits to ATG14- or UVRAG-containing complexes with BECN1:UVRAG being more stable than BECN1:ATG14 (By similarity).

  • SwissProt ID

    Q14457

  • Gene ID
    8678 [NCBI]
  • Synonyms

    Beclin-1; ATG6; beclin1; VPS30; Beclin 1 like protein 1; 4921513J16Rik; 5430417M23Rik; ATG6; BECLIN 1; BECN1; MGC6843; BECN1_HUMAN.

Beclin 1 Antibody (YA6368) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa(lane 2(20μg) , HepG2(lane 3(20μg) ,NIH-3T3(lane 4(20μg)and C6(lane 5(20μg) using Beclin 1 Antibody（HY-P86676）.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • Beclin 1 Antibody (YA6368) Product Image
    1/16
  • Experimental Validation Results for Beclin 1 Antibody (YA6368)
  • Beclin 1 Antibody (YA6368) Product Image
  • Experimental Validation Results for Beclin 1 Antibody (YA6368)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa(lane 2(20μg) , HepG2(lane 3(20μg) ,NIH-3T3(lane 4(20μg)and C6(lane 5(20μg) using Beclin 1 Antibody（HY-P86676）.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Beclin 1 Antibody (YA6368)
  • Beclin 1 Antibody (YA6368) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa(lane 2(20μg) , HepG2(lane 3(20μg) ,NIH-3T3(lane 4(20μg)and C6(lane 5(20μg) using Beclin 1 Antibody（HY-P86676）.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • Beclin 1 Antibody (YA6368) Product Image WB