Importin 9/RANBP9 Antibody (YA1625)
(Synonyms: importin 9; Imp9; Ran-binding protein 9; RanBP9)Importin 9/RANBP9 Antibody (YA1625) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Importin 9/RANBP9.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, IP
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Reactivity :
Human
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
|
|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:200 | 1:20 |
Product Details
Importin 9/RANBP9 Antibody (YA1625) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Importin 9/RANBP9.
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Host Rabbit
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Clonality Recombinant, Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 116 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 116 kDa
A synthetic peptide of human Importin 9
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Importin 9/RANBP9 is a Nuclear transport receptor that mediates nuclear import of proteins, such as histones, proteasome and actin. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the import of pre-assembled proteasomes into the nucleus; AKIRIN2 acts as a molecular bridge between IPO9 and the proteasome complex. Mediates the nuclear import of histones H2A, H2B, H4 and H4. In addition to nuclear import, also acts as a chaperone for histones by preventing inappropriate non-nucleosomal interactions. Mediates the nuclear import of actin[1][2][3].
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Subcellular Localization
Cytoplasm; Nucleus
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Subunit
Interacts with histones H2A, H2B, H3 and H4 (PubMed:11823430, PubMed:30855230). The binding is coupled to RanGTP cycles (PubMed:11823430). Interacts with AKIRIN2; promoting association with pre-assembled proteasomes (PubMed:34711951). Associates with pre-assembled proteasomes; interaction is indirect and mediated via interaction with AKIRIN2 (PubMed:34711951). Interacts with PPP2R1A and PPP2R1B (PubMed:12670497)
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SwissProt ID
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Synonyms
importin 9; Imp9; Ran-binding protein 9; RanBP9
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Research Field
Signal Transduction
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