PIK3C2A Antibody (YA8242)(PBS only)

Cat. No.: HY-P88558A: Ficha de datos User Guide for Antibodies
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PIK3C2A Antibody (YA8242) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PIK3C2A.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn
Referencias

Descripciòn

PIK3C2A Antibody (YA8242) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PIK3C2A.

Host

Mouse

Peso molecular

Predicted band size: 190.5 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O00443

Immunogen

Human recombinant protein fragment corresponding to amino acids 230-560 of human PIK3C2A produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Pureza	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion. Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin-mediated RHOQ activation. Regulates insulin secretion through two different mechanisms: involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion. Synthesizes PtdIns3P in response to insulin signaling. Functions in clathrin-coated endocytic vesicle formation and distribution. Regulates dynamin-independent endocytosis, probably by recruiting EEA1 to internalizing vesicles. In neurosecretory cells synthesizes PtdIns3P on large dense core vesicles. Participates in calcium induced contraction of vascular smooth muscle by regulating myosin light chain (MLC) phosphorylation through a mechanism involving Rho kinase-dependent phosphorylation of the MLCP-regulatory subunit MYPT1. May play a role in the EGF signaling cascade. May be involved in mitosis and UV-induced damage response. Required for maintenance of normal renal structure and function by supporting normal podocyte function. Involved in the regulation of ciliogenesis and trafficking of ciliary components (PubMed:31034465)

Subcellular Localization：Cell membrane,Cytoplasmic vesicle, clathrin-coated vesicle,Nucleus,Cytoplasm,Golgi apparatus, trans-Golgi network

Expression：
Tissue_Specificity: Expressed in columnar and transitional epithelia, mononuclear cells, smooth muscle cells, and endothelial cells lining capillaries and small venules (at protein level). Ubiquitously expressed, with highest levels in heart, placenta and ovary, and lowest levels in the kidney. Detected at low levels in islets of Langerhans from type 2 diabetes mellitus individuals

Isoforms & Post-Translational Modification：O00443 has two isomers: O00443-1: 190680 Da (predicted); O00443-2: 53742 Da (predicted).
Phosphorylated upon insulin stimulation; which may lead to enzyme activation (By similarity)

Subunit：Part of a complex with ERBB2 and EGFR (PubMed:10805725)

Synonyms

CPK; PI3-K-C2(ALPHA); PI3-K-C2A

Documentación

User Guide for Antibodies (1077 KB)

Referencias

[1]. /biology-dictionary/mda-mb-231-cell.html [Content Brief]