Dopamine Receptor D4 Antibody
(Synonyms: D(4) dopamine receptor, D(2C) dopamine receptor, Dopamine D4 receptor, DRD4)Dopamine Receptor D4 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Dopamine Receptor D4.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
Dopamine Receptor D4 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Dopamine Receptor D4.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 48 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 43 kDa
Synthetic peptide corresponding to the C-term region of human Dopamine Receptor D4.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Dopamine Receptor D4 is a Dopamine receptor responsible for neuronal signaling in the mesolimbic system of the brain, an area of the brain that regulates emotion and complex behavior. Activated by dopamine, but also by epinephrine and norepinephrine, and by numerous synthetic agonists and drugs. Agonist binding triggers signaling via G proteins that inhibit adenylyl cyclase. Modulates the circadian rhythm of contrast sensitivity by regulating the rhythmic expression of NPAS2 in the retinal ganglion cells (By similarity)[1][2][3][4][5][6].
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Subcellular Localization
Cell membrane
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Expression
Tissue_Specificity: Highly expressed in retina. Detected at much lower levels in brain, in amygdala, thalamus, hypothalamus, cerebellum and pituitary. -
Isoforms & Post-Translational Modification
Dopamine Receptor D4 has an amino acid length of 419, molecular weight is 43901 Da.
Polyubiquitinated by the BCR(KLHL12) E3 ubiquitin ligase complex: polyubiquitination does not lead to degradation of DRD4 protein. -
Subunit
Forms homo- and heterooligomers with DRD2.
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SwissProt ID
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Synonyms
D(4) dopamine receptor, D(2C) dopamine receptor, Dopamine D4 receptor, DRD4
Documentation
[1]. Czermak C, et al. [35S]GTPgammaS binding at the human dopamine D4 receptor variants hD4.2, hD4.4 and hD4.7 following stimulation by dopamine, epinephrine and norepinephrine. Eur J Pharmacol. 2006 Feb 15;531(1-3):20-4. [Content Brief]
[2]. Zhang X, et al. Palmitoylation of the carboxyl-terminal tail of dopamine D(4) receptor is required for surface expression, endocytosis, and signaling. Biochem Biophys Res Commun. 2016 Oct 14;479(2):398-403. [Content Brief]
[3]. Wang S, et al. D(4) dopamine receptor high-resolution structures enable the discovery of selective agonists. Science. 2017 Oct 20;358(6361):381-386. [Content Brief]
[4]. Lanau F, et al. Epinephrine and norepinephrine act as potent agonists at the recombinant human dopamine D4 receptor. J Neurochem. 1997 Feb;68(2):804-12. [Content Brief]
[5]. Chio CL, et al. D4 dopamine receptor-mediated signaling events determined in transfected Chinese hamster ovary cells. J Biol Chem. 1994 Apr 22;269(16):11813-9. [Content Brief]
[6]. Asghari V, et al. Modulation of intracellular cyclic AMP levels by different human dopamine D4 receptor variants. J Neurochem. 1995 Sep;65(3):1157-65. [Content Brief]
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