RAB11A Antibody (YA10062)
(Synonyms: RAB11, RAB11A, Ras-related protein Rab-11A, Rab-11, YL8)RAB11A Antibody (YA10062) is a Mouse-derived and non-conjugated IgG1 Monoclonal antibody, targeting to RAB11A.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
RAB11A Antibody (YA10062) is a Mouse-derived and non-conjugated IgG1 Monoclonal antibody, targeting to RAB11A.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 24 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 24 kDa
Purified recombinant protein of human RAB11A.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), 293T (lane 3), SH-SY5Y (lane 4), and Raji (lane 5) using RAB11A antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
Background
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Function
RAB11A is a The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB11A regulates endocytic recycling. Forms a functional Rab11/RAB11FIP3/dynein complex that regulates the movement of peripheral sorting endosomes (SE) along microtubule tracks toward the microtubule organizing center/centrosome, generating the endosomal recycling compartment (ERC). Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with Rabin8/RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric IgG) to basolateral membranes (By similarity). May also play a role in melanosome transport and release from melanocytes (By similarity). Promotes Rabin8/RAB3IP preciliary vesicular trafficking to mother centriole by forming a ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, thereby regulating ciliogenesis initiation. On the contrary, upon LPAR1 receptor signaling pathway activation, interaction with phosphorylated WDR44 prevents Rab11-RAB3IP-RAB11FIP3 complex formation and cilia growth. Participates in the export of a subset of neosynthesized proteins through a Rab8-Rab10-Rab11-endososomal dependent export route via interaction with WDR44. Also interacts with RABL3 to promote ciliary vesicle formation[1][2][3][4][5][6][7][8][9][10][11][12].
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Subcellular Localization
Cell membrane; Endosome membrane; Recycling endosome membrane; Cleavage furrow; Cytoplasmic vesicle, phagosome membrane; Golgi apparatus membrane; Golgi apparatus, trans-Golgi network; Cytoplasmic vesicle membrane; Cell projection, cilium
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Isoforms & Post-Translational Modification
RAB11A has 2 isoforms, P62491-1: amino acid length is 216, molecular weight is 24394 Da (predicted); P62491-2: amino acid length is 155, molecular weight is 17659 Da (predicted).(Microbial infection) Glycosylated on arginine residues by S.typhimurium protein Ssek3
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Subunit
Interacts (GTP-bound form) with RAB11FIPs (via their C-termini) including RAB11FIP1, RAB11FIP2, RAB11FIP3, RAB11FIP4 and RAB11FIP5 effectors.
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SwissProt ID
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Synonyms
RAB11, RAB11A, Ras-related protein Rab-11A, Rab-11, YL8
Documentation
References
[1]. Wilson GM, et al. The FIP3-Rab11 protein complex regulates recycling endosome targeting to the cleavage furrow during late cytokinesis. Mol Biol Cell. 2005 Feb;16(2):849-60. [Content Brief]
[2]. Lock JG, et al. Rab11 in recycling endosomes regulates the sorting and basolateral transport of E-cadherin. Mol Biol Cell. 2005 Apr;16(4):1744-55. [Content Brief]
[3]. Swiatecka-Urban A, et al. Myosin Vb is required for trafficking of the cystic fibrosis transmembrane conductance regulator in Rab11a-specific apical recycling endosomes in polarized human airway epithelial cells. J Biol Chem. 2007 Aug 10;282(32):23725-36. [Content Brief]
[4]. Chu BB, et al. Requirement of myosin Vb.Rab11a.Rab11-FIP2 complex in cholesterol-regulated translocation of NPC1L1 to the cell surface. J Biol Chem. 2009 Aug 14;284(33):22481-22490. [Content Brief]
[5]. Horgan CP, et al. Rab11-FIP3 links the Rab11 GTPase and cytoplasmic dynein to mediate transport to the endosomal-recycling compartment. J Cell Sci. 2010 Jan 15;123(Pt 2):181-91. [Content Brief]
[6]. Bryant DM, et al. A molecular network for de novo generation of the apical surface and lumen. Nat Cell Biol. 2010 Nov;12(11):1035-45. [Content Brief]
[7]. Roland JT, et al. Rab GTPase-Myo5B complexes control membrane recycling and epithelial polarization. Proc Natl Acad Sci U S A. 2011 Feb 15;108(7):2789-94. [Content Brief]
[8]. Lall P, et al. Structure-Function Analyses of the Interactions between Rab11 and Rab14 Small GTPases with Their Shared Effector Rab Coupling Protein (RCP). J Biol Chem. 2015 Jul 24;290(30):18817-32. [Content Brief]
[9]. Wang J, et al. The Arf and Rab11 effector FIP3 acts synergistically with ASAP1 to direct Rabin8 in ciliary receptor targeting. J Cell Sci. 2015 Apr 1;128(7):1375-85. [Content Brief]
[10]. Walia V, et al. Akt Regulates a Rab11-Effector Switch Required for Ciliogenesis. Dev Cell. 2019 Jul 22;50(2):229-246.e7. [Content Brief]
[11]. Lucken-Ardjomande Häsler S, et al. GRAF2, WDR44, and MICAL1 mediate Rab8/10/11-dependent export of E-cadherin, MMP14, and CFTR ΔF508. J Cell Biol. 2020 May 4;219(5):. [Content Brief]
[12]. Kobayashi T, et al. The atypical small GTPase RABL3 interacts with RAB11 to regulate early ciliogenesis in human cells. J Cell Sci. 2022 Sep 15;135(18):. [Content Brief]
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