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Cathepsin K Antibody

(Synonyms: Cathepsin K; Procathepsin K; Cathepsin K precursor; Cathepsin O; Cathepsin O1; Cathepsin O2; Cathepsin X; CathepsinK; CTS02; CTSK; CTSK protein; CTSO; CTSO1; CTSO2; MGC23107; PKND; PYCD; Pycnodysostosis; cath-K; CK.)
1 Cited Publications
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Based on 1 publication(s) in Google Scholar

Cathepsin K Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Cathepsin K.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ELISA, IHC-P, IHC-F, ICC/IF

  • Reactivity :

    Rat

  • Formulation:

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Cathepsin K Antibody
  • Cathepsin K Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-2000 1:5000-10000 1:100-500 1:100-500 1:100-500

Product Details

Description

Cathepsin K Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Cathepsin K.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Rat Predicted Reactivity: Human,Mouse,Dog,Pig,Cow,Horse,GuineaPig
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 46 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 36 kDa
Species Reactivity Database

Entrez Gene: 29175 Rat

SwissProt: O35186 Rat

Immunogen

KLH conjugated synthetic peptide derived from human Cathepsin K: 112-210/329

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103080

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cathepsin K Antibody
    Western blot analysis of extracts from Heart (lane 1) and Muscle (lane 2) using Cathepsin K antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

  • Function

    Cathepsin K (CTSK) is a lysosomal cysteine protease highly expressed by bone-resorbing osteoclasts and shows potent collagenase activity in bone matrix degradation[1]. Mechanistically, CTSK supports osteoclastic resorption because it cleaves collagen I, whereas cathepsins B, S, and L show weaker or non-detected roles in osteoclast bone resorption contexts[2]. Heparan sulfate regulates CTSK by forming stable complexes, inducing dimerization, preserving peptidase activity, and selectively inhibiting collagenase activity without blocking the active site[3]. In osteoarthritis research, CTSK links to cartilage degradation through type II collagen cleavage, with increased serum CTSK in patients and upregulated CTSK in degraded cartilage in animal models[4]. In periodontitis models, CTSK inhibition with odanacatib reduced osteoclasts, macrophages, T cells, Toll-like receptor expression, bone loss, and immune activation[5]. Compared with related isoforms, CTSK has distinctive collagenolytic activity and selectivity challenges because homologous CTSK/S binding sites can cause unselective inhibition[6]. For experimental applications, CTSK inhibitors, heparan sulfate oligosaccharides, and light-activated inhibitors provide tools to study bone resorption, cartilage degradation, and collagen I degradation in 3D tumor spheroids[3][4][7].

  • Subcellular Localization

    Lysosome; Secreted; Apical cell membrane; Peripheral membrane protein; Extracellular side

  • Expression


    Tissue_specificity:It is primarily expressed in osteoclasts (skeletal tissue) (PubMed:7805878) . It is also expressed in thyroid epithelial cells (PubMed:11082042) .

  • SwissProt ID

    P43235

  • Gene ID
    1513 [NCBI]
  • Synonyms

    Cathepsin K; Procathepsin K; Cathepsin K precursor; Cathepsin O; Cathepsin O1; Cathepsin O2; Cathepsin X; CathepsinK; CTS02; CTSK; CTSK protein; CTSO; CTSO1; CTSO2; MGC23107; PKND; PYCD; Pycnodysostosis; cath-K; CK.

[1]. Guan X, et al. Small-molecule Cathepsin K inhibitors: a medicinal chemistry perspective. Future Med Chem. 2025 Jul;17(14):1757-1770. [Content Brief]

[2]. Zaidi M, et al. Cathepsin K, osteoclastic resorption, and osteoporosis therapy. J Bone Miner Res. 2001 Oct;16(10):1747-9. [Content Brief]

[3]. Zhang X, et al. Heparan sulfate selectively inhibits the collagenase activity of cathepsin K. Matrix Biol. 2024 May;129:15-28. [Content Brief]

[4]. Brizuela L, et al. Cathepsin K Inhibitors as Potential Drugs for the Treatment of Osteoarthritis. Int J Mol Sci. 2025 Mar 22;26(7):2896. [Content Brief]

[5]. Hao L, et al. Odanacatib, A Cathepsin K-Specific Inhibitor, Inhibits Inflammation and Bone Loss Caused by Periodontal Diseases. J Periodontol. 2015 Aug;86(8):972-83. [Content Brief]

[6]. Khalili M, et al. Underserved Does Not Mean Undeserved: Unfurling the HCV Care in the Safety Net. Dig Dis Sci. 2018 Dec;63(12):3250-3252. [Content Brief]

[7]. Herroon MK, et al. Photoactivated inhibition of cathepsin K in a 3D tumor model. Biol Chem. 2016 Jun 1;397(6):571-82. [Content Brief]

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Cathepsin K Antibody Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Heart (lane 1) and Muscle (lane 2) using Cathepsin K antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • Cathepsin K Antibody Product Image
    1/16
  • Experimental Validation Results for Cathepsin K Antibody
  • Cathepsin K Antibody Product Image
  • Experimental Validation Results for Cathepsin K Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Heart (lane 1) and Muscle (lane 2) using Cathepsin K antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Cathepsin K Antibody
  • Cathepsin K Antibody Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Heart (lane 1) and Muscle (lane 2) using Cathepsin K antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • Cathepsin K Antibody Product Image WB