SIRT5 Antibody (YA079)

製品番号: HY-P80321: データシート SDS COA User Guide for Antibodies
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SIRT5 Antibody (YA079) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SIRT5.

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容量	価格（税別）	在庫状況	数量
無料サンプル		今すぐ申し込む
10 μL	$84	在庫あり	Estimated Time of Arrival: December 31
50 μL	$222	在庫あり	Estimated Time of Arrival: December 31
100 μL	$360	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明
参考文献

製品説明

SIRT5 Antibody (YA079) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SIRT5.

Host

Rabbit

Clonality

Recombinant, Monoclonal

分子量

Predicted band size: 34 kDa;

Observed band size: 30 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9NXA8

Gene ID

23408 [NCBI]

Immunogen

Synthetic peptide corresponding to Human SIRT5.AA range:1-160.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000

Sensitivity	Endogenous	純度	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HepG2 (lane 2(20μg), HepG2 (lane 3(40μg), using SIRT5 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed:21908771, PubMed:22076378, PubMed:24703693, PubMed:29180469). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed:22076378, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed:24140062). Activates SHMT2 by mediating its desuccinylation (PubMed:29180469). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX

Subcellular Localization：Mitochondrion matrix; Mitochondrion intermembrane space; Cytoplasm, cytosol; Nucleus; Cytoplasm; Mitochondrion; Mitochondrion

Expression：
Tissue_specificity:Widely expressed

Isoforms & Post-Translational Modification：Q9NXA8 has 4 isomers: Q9NXA8-1: 33881 Da (predicted); Q9NXA8-2: 32674 Da (predicted); Q9NXA8-3: 31994 Da (predicted); Q9NXA8-4: 21689 Da (predicted).

Subunit：Interacts with CPS1 (By similarity). Interacts with PCCA (PubMed:23438705). Monomer (PubMed:17355872). Homodimer (PubMed:17355872). Forms homodimers upon suramin binding (PubMed:17355872)

RRID

AB_3102887

Database

Entrez Gene: 23408 Human

SwissProt: Q9NXA8 Human

OMIM: 604483 Human

Research Field

Epigenetics and Nuclear Signaling

ドキュメンテーション

Select Batch:

データシート (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

参考文献

[1]. /biology-dictionary/plc-prf-5-cell.html [Content Brief]