Phospho-Acetyl Coenzyme A Carboxylase (Ser79) Antibody (YA229)

製品番号: HY-P80787: データシート SDS COA User Guide for Antibodies
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Phospho-Acetyl Coenzyme A Carboxylase (Ser79) Antibody (YA229) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Acetyl Coenzyme A Carboxylase (Ser79).

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容量	価格（税別）	在庫状況	数量
10 μL	$94	在庫あり	Estimated Time of Arrival: December 31
50 μL	$249	在庫あり	Estimated Time of Arrival: December 31
100 μL	$405	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

Phospho-Acetyl Coenzyme A Carboxylase (Ser79) Antibody (YA229) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Acetyl Coenzyme A Carboxylase (Ser79).

Host

Rabbit

Clonality

Recombinant,Monoclonal

分子量

Predicted band size: 266 kDa;

Observed band size: 266 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q13085

Gene ID

31 [NCBI]

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser79 of Human Acetyl Coenzyme A Carboxylase.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000

Sensitivity	Endogenous	純度	affinity purified
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG

Appearance

Solution

Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from K562(lane 2(20ug) and K562(lane 3(40ug) using Phospho-Acetyl Coenzyme A Carboxylase Antibody (HY-P80787) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis (PubMed:20457939, PubMed:20952656, PubMed:29899443). This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed:20457939, PubMed:20952656, PubMed:29899443)

Subcellular Localization：Cytoplasm, cytosol

Expression：
Tissue_specificity:It is expressed in the brain, placenta, skeletal muscle, kidney, pancreas, and adipose tissue; it is expressed at low levels in lung tissue; and it was not detected in the liver.

Isoforms & Post-Translational Modification：Q13085 has 4 isomers: Q13085-1: 265554 Da (predicted); Q13085-2: 259686 Da (predicted); Q13085-3: 257239 Da (predicted); Q13085-4: 269999 Da (predicted).
Phosphorylation on Ser-1263 is required for interaction with BRCA1;Phosphorylation at Ser-80 by AMPK inactivates enzyme activity;The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity

Subunit：Monomer, homodimer, and homotetramer (PubMed:20952656, PubMed:29899443). Can form filamentous polymers (PubMed:20457939, PubMed:20952656, PubMed:29899443). Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis (PubMed:12360400, PubMed:16326698, PubMed:18452305, PubMed:29899443). Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity (PubMed:20457939)

RRID

AB_3102712

Database

Entrez Gene: 31 Human

SwissProt: Q13085 Human

OMIM: 613933 Human

Research Field

Cardiovascular

Synonyms

ACACA; ACAC; ACC1; ACCA; Acetyl-CoA carboxylase 1; ACC1; ACC-alpha

ドキュメンテーション

Select Batch:

データシート (235 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)