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Phospho-Acetyl Coenzyme A Carboxylase (Ser79) Antibody (YA229)

(Synonyms: ACACA; ACAC; ACC1; ACCA; Acetyl-CoA carboxylase 1; ACC1; ACC-alpha)
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Based on 1 Customer Validation

Phospho-Acetyl Coenzyme A Carboxylase (Ser79) Antibody (YA229) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Acetyl Coenzyme A Carboxylase (Ser79).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospho-Acetyl Coenzyme A Carboxylase (Ser79) Antibody (YA229)
  • Phospho-Acetyl Coenzyme A Carboxylase (Ser79) Antibody (YA229) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000-1:5000

Product Details

Description

Phospho-Acetyl Coenzyme A Carboxylase (Ser79) Antibody (YA229) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Acetyl Coenzyme A Carboxylase (Ser79).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 266 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 266 kDa
Species Reactivity Database

Entrez Gene: 31 Human

SwissProt: Q13085 Human

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser79 of Human Acetyl Coenzyme A Carboxylase.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3102712

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-Acetyl Coenzyme A Carboxylase (Ser79) Antibody (YA229)
    Western blot analysis of extracts from K562(lane 2(20ug) and K562(lane 3(40ug) using Phospho-Acetyl Coenzyme A Carboxylase Antibody (HY-P80787) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Acetyl Coenzyme A Carboxylase is a Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis. This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA[1][2][3].

  • Subcellular Localization

    Cytoplasm, cytosol

  • Expression


    Tissue_specificity:It is expressed in the brain, placenta, skeletal muscle, kidney, pancreas, and adipose tissue; it is expressed at low levels in lung tissue; and it was not detected in the liver.

  • Isoforms & Post-Translational Modification

    Q13085 has 4 isomers: Q13085-1: 265554 Da (predicted); Q13085-2: 259686 Da (predicted); Q13085-3: 257239 Da (predicted); Q13085-4: 269999 Da (predicted).
    Phosphorylation on Ser-1263 is required for interaction with BRCA1;Phosphorylation at Ser-80 by AMPK inactivates enzyme activity;The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity

  • Subunit

    Monomer, homodimer, and homotetramer (PubMed:20952656, PubMed:29899443). Can form filamentous polymers (PubMed:20457939, PubMed:20952656, PubMed:29899443). Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis (PubMed:12360400, PubMed:16326698, PubMed:18452305, PubMed:29899443). Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity (PubMed:20457939)

  • SwissProt ID

    Q13085

  • Gene ID
    31 [NCBI]
  • Synonyms

    ACACA; ACAC; ACC1; ACCA; Acetyl-CoA carboxylase 1; ACC1; ACC-alpha

  • Research Field

    Cardiovascular

References

[1]. Kim CW, et al. Induced polymerization of mammalian acetyl-CoA carboxylase by MIG12 provides a tertiary level of regulation of fatty acid synthesis. Proc Natl Acad Sci U S A. 2010 May 25;107(21):9626-31. [Content Brief]

[2]. Colbert CL, et al. Crystal structure of Spot 14, a modulator of fatty acid synthesis. Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):18820-5. [Content Brief]

[3]. Hunkeler M, et al. Structural basis for regulation of human acetyl-CoA carboxylase. Nature. 2018 Jun;558(7710):470-474. [Content Brief]

Phospho-Acetyl Coenzyme A Carboxylase (Ser79) Antibody (YA229) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug) and K562(lane 3(40ug) using Phospho-Acetyl Coenzyme A Carboxylase Antibody (HY-P80787) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Phospho-Acetyl Coenzyme A Carboxylase (Ser79) Antibody (YA229) Product Image
    1/16
  • Experimental Validation Results for Phospho-Acetyl Coenzyme A Carboxylase (Ser79) Antibody (YA229)
  • Phospho-Acetyl Coenzyme A Carboxylase (Ser79) Antibody (YA229) Product Image
  • Experimental Validation Results for Phospho-Acetyl Coenzyme A Carboxylase (Ser79) Antibody (YA229)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug) and K562(lane 3(40ug) using Phospho-Acetyl Coenzyme A Carboxylase Antibody (HY-P80787) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-Acetyl Coenzyme A Carboxylase (Ser79) Antibody (YA229)
  • Phospho-Acetyl Coenzyme A Carboxylase (Ser79) Antibody (YA229) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562(lane 2(20ug) and K562(lane 3(40ug) using Phospho-Acetyl Coenzyme A Carboxylase Antibody (HY-P80787) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Phospho-Acetyl Coenzyme A Carboxylase (Ser79) Antibody (YA229) Product Image WB