GRASP55 Antibody
(Synonyms: GOLPH6, GORASP2, Golgi reassembly-stacking protein 2, GRS2, Golgi phosphoprotein 6, Golgi reassembly-stacking protein of 55 kDa, p59, GRASP55)GRASP55 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to GRASP55.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
GRASP55 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to GRASP55.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 55 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 47 kDa
Synthetic peptide corresponding to the center region of human GRASP55.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
GRASP55 is key structural protein of the Golgi apparatus. The membrane cisternae of the Golgi apparatus adhere to each other to form stacks, which are aligned side by side to form the Golgi ribbon. Acting in concert with GORASP1/GRASP65, is required for the formation and maintenance of the Golgi ribbon, and may be dispensable for the formation of stacks. However, other studies suggest that GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after breakdown during mitosis and meiosis. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA. Required for normal acrosome formation during spermiogenesis and normal male fertility, probably by promoting colocalization of JAM2 and JAM3 at contact sites between germ cells and Sertoli cells (By similarity). Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane[1][2][3][4][5][6][7][8].
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Subcellular Localization
Golgi apparatus membrane; Endoplasmic reticulum membrane; Golgi apparatus
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Isoforms & Post-Translational Modification
GRASP55 has 3 isoforms, Q9H8Y8-1: amino acid length is 452, molecular weight is 47145 Da (predicted); Q9H8Y8-2: amino acid length is 384, molecular weight is 39768 Da (predicted); Q9H8Y8-3: amino acid length is 464, molecular weight is 48674 Da (predicted).发生豆蔻酰化修饰(PubMed:11101516)。豆蔻酰化是高尔基体靶向所必需的(根据相似性推断)GRASP55 存在 3 个异构体，Q9H8Y8-1：氨基酸个数为 452 个，分子量为 47145 Da (预测)；Q9H8Y8-2：氨基酸个数为 384 个，分子量为 39768 Da (预测)；Q9H8Y8-3：氨基酸个数为 464 个，分子量为 48674 Da (预测)。Myristoylated (PubMed:11101516). Myristoylation is essential for the Golgi targeting (By similarity)
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Subunit
Homodimer.
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SwissProt ID
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Synonyms
GOLPH6, GORASP2, Golgi reassembly-stacking protein 2, GRS2, Golgi phosphoprotein 6, Golgi reassembly-stacking protein of 55 kDa, p59, GRASP55
Documentation
[1]. Zhang Y, et al. Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure. J Cell Biol. 2021 Jan 4;220(1):. [Content Brief]
[2]. Shorter J, et al. GRASP55, a second mammalian GRASP protein involved in the stacking of Golgi cisternae in a cell-free system. EMBO J. 1999 Sep 15;18(18):4949-60. [Content Brief]
[3]. Truschel ST, et al. Structure of the membrane-tethering GRASP domain reveals a unique PDZ ligand interaction that mediates Golgi biogenesis. J Biol Chem. 2011 Jun 10;286(23):20125-9. [Content Brief]
[4]. Truschel ST, et al. Allosteric regulation of GRASP protein-dependent Golgi membrane tethering by mitotic phosphorylation. J Biol Chem. 2012 Jun 8;287(24):19870-5. [Content Brief]
[5]. Kuo A, et al. Transmembrane transforming growth factor-alpha tethers to the PDZ domain-containing, Golgi membrane-associated protein p59/GRASP55. EMBO J. 2000 Dec 1;19(23):6427-39. [Content Brief]
[6]. Gee HY, et al. Rescue of ΔF508-CFTR trafficking via a GRASP-dependent unconventional secretion pathway. Cell. 2011 Sep 2;146(5):746-60. [Content Brief]
[7]. Kim J, et al. Monomerization and ER Relocalization of GRASP Is a Requisite for Unconventional Secretion of CFTR. Traffic. 2016 Jul;17(7):733-53. [Content Brief]
[8]. Piao H, et al. Sec16A is critical for both conventional and unconventional secretion of CFTR. Sci Rep. 2017 Jan 9;7:39887. [Content Brief]
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