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  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. SLC12A1 Antibody (YA3471)

SLC12A1 Antibody (YA3471)

Cat. No.: HY-P83742
User Guide for Antibodies Technical Support

SLC12A1 Antibody (YA3471) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SLC12A1.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

Description

SLC12A1 Antibody (YA3471) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SLC12A1.
Host

Rabbit
Clonality

Monoclonal, Recombinant
Molecular Weight
Predicted band size: 121 kDa;
Observed band size: 150 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

Recombinant protein within Human SLC12A1 / NKCC2 aa 593-813 / 1,099.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:2000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:20000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:100
mIHC
mIHC: Multiplex Immunohistochemical
1:3000
Purity Affinity Purified Conjugation Non-conjugated
Modification Unmodified Isotype IgG
Appearance

Liquid
Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：Renal sodium, potassium and chloride ion cotransporter that mediates the transepithelial NaCl reabsorption in the thick ascending limb and plays an essential role in the urinary concentration and volume regulation (PubMed:21321328). Electrically silent transporter system (By similarity)
Subcellular Localization：Apical cell membrane; Multi-pass membrane protein
Expression：
Tissue_specificity:Kidney; located in the thick ascending limb of the renal tubule (protein level) .
Positive sample: Mouse kidney tissue lysate, rat kidney tissue lysate, K-562 cell lysate, mouse kidney tissue, K-562.
Isoforms & Post-Translational Modification：Q13621 has 3 isomers: Q13621-1: 121450 Da (predicted); Q13621-2: Da (predicted); Q13621-3: 121388 Da (predicted).
Phosphorylated at Ser-91, Thr-100 and Thr-105 by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4), promoting its activity
Subunit：When phosphorylated, interacts with PPP3CB
Database

Entrez Gene: 6557 Human ; 20495 Mouse ;

SwissProt: Q13621 Human ; P55014 Mouse ; P55016 Rat

OMIM: 601678 Human

Research Field

Signal Transduction
Synonyms
BSC1 antibody; Bumetanide sensitive sodium 3 antibody; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 antibody; Kidney specific Na K Cl symporter antibody; Kidney-specific Na-K-Cl symporter antibody; MGC48843 antibody; Na K 2Cl cotransporter antibody; NKCC2 antibody; potassiumchloride cotransporter 2 antibody; S12A1_HUMAN antibody; Slc12a1 antibody; sodium potassium chloride cotransporter 2 antibody; solute carrier family 12 (sodium/potassium/chloride transporters) antibody; Solute carrier family 12 member 1 antibody
Documentation
User Guide for Antibodies (1077 KB)

SLC12A1 Antibody (YA3471) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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