SLC12A1 Antibody (YA3471)

製品番号: HY-P83742: データシート User Guide for Antibodies
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SLC12A1 Antibody (YA3471) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SLC12A1.

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容量		在庫状況
50 μL		お問い合わせ
100 μL		お問い合わせ

* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
説明

製品説明

SLC12A1 Antibody (YA3471) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SLC12A1.

Host

Rabbit

Clonality

Monoclonal, Recombinant

分子量

Predicted band size: 121 kDa;

Observed band size: 150 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q13621

Gene ID

6557 [NCBI]

Immunogen

Recombinant protein within Human SLC12A1 / NKCC2 aa 593-813 / 1,099.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:20000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
mIHC mIHC: Multiplex Immunohistochemical	1:3000

純度	Affinity Purified	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Background

Function：Renal sodium, potassium and chloride ion cotransporter that mediates the transepithelial NaCl reabsorption in the thick ascending limb and plays an essential role in the urinary concentration and volume regulation (PubMed:21321328). Electrically silent transporter system (By similarity)

Subcellular Localization：Apical cell membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Kidney; located in the thick ascending limb of the renal tubule (protein level) .
Positive sample: Mouse kidney tissue lysate, rat kidney tissue lysate, K-562 cell lysate, mouse kidney tissue, K-562.

Isoforms & Post-Translational Modification：Q13621 has 3 isomers: Q13621-1: 121450 Da (predicted); Q13621-2: Da (predicted); Q13621-3: 121388 Da (predicted).
Phosphorylated at Ser-91, Thr-100 and Thr-105 by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4), promoting its activity

Subunit：When phosphorylated, interacts with PPP3CB

Database

Entrez Gene: 6557 Human ; 20495 Mouse ;

SwissProt: Q13621 Human ; P55014 Mouse ; P55016 Rat

OMIM: 601678 Human

Research Field

Signal Transduction

Synonyms

BSC1 antibody; Bumetanide sensitive sodium 3 antibody; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 antibody; Kidney specific Na K Cl symporter antibody; Kidney-specific Na-K-Cl symporter antibody; MGC48843 antibody; Na K 2Cl cotransporter antibody; NKCC2 antibody; potassiumchloride cotransporter 2 antibody; S12A1_HUMAN antibody; Slc12a1 antibody; sodium potassium chloride cotransporter 2 antibody; solute carrier family 12 (sodium/potassium/chloride transporters) antibody; Solute carrier family 12 member 1 antibody

ドキュメンテーション

User Guide for Antibodies (1077 KB)