Phospho-NFAT2 (Ser237) Antibody (YA1389)
(Synonyms: NFATC1; NFAT2; NFATC; Nuclear factor of activated T-cells; cytoplasmic 1; NF-ATc1; NFATc1; NFAT transcription complex cytosolic component; NF-ATc; NFATc)Phospho-NFAT2 (Ser237) Antibody (YA1389) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-NFAT2 (Ser237).
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human, Mouse
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
Phospho-NFAT2 (Ser237) Antibody (YA1389) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-NFAT2 (Ser237).
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Host Rabbit
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Clonality Recombinant, Monoclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 101 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 101 kDa
A synthetic phosphopeptide corresponding to residues surrounding Ser237 of human NFAT2
Endogenous
Affinity Purified
Non-conjugated
Phosphorylated
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
NFAT2 plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells. Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function[1].
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Subcellular Localization
Cytoplasm; Nucleus
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Expression
Tissue_specificity:This protein is expressed in the thymus, peripheral blood leukocytes (such as T cells) , and spleen. The A subtype is preferentially expressed in effector T cells (thymus and peripheral blood leukocytes) , while the B and C subtypes are preferentially expressed in naive T cells (spleen) . The B subtype is expressed after the first exposure of naive T cells to the antigen, and the A subtype is expressed after the second exposure of effector T cells to the antigen. The IA subtype is widely expressed but not detected in the liver and pancreas; its neural expression is strongest in the corpus callosum. The IB subtype is mainly expressed in muscle, cerebellum, placenta, and thymus; neural expression is present in all of these areas of the human brain, with the strongest expression in the corpus callosum.
Induction:Only isoforms A are inducibly expressed in T lymphocytes upon activation of the T-cell receptor (TCR) complex. Induced after co-addition of phorbol 12-myristate 13-acetate (PMA) and ionomycin. Also induced after co-addition of 12-O-tetradecanoylphorbol-13-acetate (TPA) and ionomycin. Weakly induced with PMA, ionomycin and cyclosporin A -
Isoforms & Post-Translational Modification
O95644 has 10 isomers: O95644-1: 101243 Da (predicted); O95644-2: 77785 Da (predicted); O95644-3: 76879 Da (predicted); O95644-4: 88763 Da (predicted); O95644-5: 87857 Da (predicted); O95644-6: 100336 Da (predicted); O95644-8: 74276 Da (predicted); O95644-10: 77376 Da (predicted); O95644-11: 76469 Da (predicted); O95644-17: 38626 Da (predicted).
Phosphorylated by NFATC-kinase and GSK3B; phosphorylation induces NFATC1 nuclear exit and dephosphorylation by calcineurin promotes nuclear import. Phosphorylation by PKA and DYRK2 negatively modulates nuclear accumulation, and promotes subsequent phosphorylation by GSK3B or casein kinase 1 -
Subunit
Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. NFATC proteins bind to DNA as monomers (PubMed:9506523). Interacts with HOMER2 and HOMER3; this interaction may compete with calcineurin/PPP3CA-binding and hence prevent NFATC1 dephosphorylation and activation (PubMed:18218901). Interacts with TLE6/GRG6 (By similarity)
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SwissProt ID
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Synonyms
NFATC1; NFAT2; NFATC; Nuclear factor of activated T-cells; cytoplasmic 1; NF-ATc1; NFATc1; NFAT transcription complex cytosolic component; NF-ATc; NFATc
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Research Field
Immunology
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