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  5. Phospho-NFAT2 (Ser237) Antibody (YA1389)

Phospho-NFAT2 (Ser237) Antibody (YA1389)

Cat. No.: HY-P81644
User Guide for Antibodies Technical Support

Phospho-NFAT2 (Ser237) Antibody (YA1389) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-NFAT2 (Ser237).

For research use only. We do not sell to patients.

Size Price Stock Quantity
20 μL Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
50 μL Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
100 μL Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
250 μL   Get quote  
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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

Description

Phospho-NFAT2 (Ser237) Antibody (YA1389) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-NFAT2 (Ser237).
Host

Rabbit
Clonality

Recombinant, Monoclonal
Molecular Weight
Predicted band size: 101 kDa;
Observed band size: 101 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse
SwissProt ID
Gene ID
Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser237 of human NFAT2
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Phosphorylated
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells (PubMed:10358178). Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function (By similarity)
Subcellular Localization：Cytoplasm; Nucleus
Expression：
Tissue_specificity:This protein is expressed in the thymus, peripheral blood leukocytes (such as T cells) , and spleen. The A subtype is preferentially expressed in effector T cells (thymus and peripheral blood leukocytes) , while the B and C subtypes are preferentially expressed in naive T cells (spleen) . The B subtype is expressed after the first exposure of naive T cells to the antigen, and the A subtype is expressed after the second exposure of effector T cells to the antigen. The IA subtype is widely expressed but not detected in the liver and pancreas; its neural expression is strongest in the corpus callosum. The IB subtype is mainly expressed in muscle, cerebellum, placenta, and thymus; neural expression is present in all of these areas of the human brain, with the strongest expression in the corpus callosum.

Induction:Only isoforms A are inducibly expressed in T lymphocytes upon activation of the T-cell receptor (TCR) complex. Induced after co-addition of phorbol 12-myristate 13-acetate (PMA) and ionomycin. Also induced after co-addition of 12-O-tetradecanoylphorbol-13-acetate (TPA) and ionomycin. Weakly induced with PMA, ionomycin and cyclosporin A
Isoforms & Post-Translational Modification：O95644 has 10 isomers: O95644-1: 101243 Da (predicted); O95644-2: 77785 Da (predicted); O95644-3: 76879 Da (predicted); O95644-4: 88763 Da (predicted); O95644-5: 87857 Da (predicted); O95644-6: 100336 Da (predicted); O95644-8: 74276 Da (predicted); O95644-10: 77376 Da (predicted); O95644-11: 76469 Da (predicted); O95644-17: 38626 Da (predicted).
Phosphorylated by NFATC-kinase and GSK3B; phosphorylation induces NFATC1 nuclear exit and dephosphorylation by calcineurin promotes nuclear import. Phosphorylation by PKA and DYRK2 negatively modulates nuclear accumulation, and promotes subsequent phosphorylation by GSK3B or casein kinase 1
Subunit：Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. NFATC proteins bind to DNA as monomers (PubMed:9506523). Interacts with HOMER2 and HOMER3; this interaction may compete with calcineurin/PPP3CA-binding and hence prevent NFATC1 dephosphorylation and activation (PubMed:18218901). Interacts with TLE6/GRG6 (By similarity)
Database

Entrez Gene: 4772 Human ;

SwissProt: O95644 Human ; O88942 Mouse ;

OMIM: 600489 Human

Research Field

Immunology
Synonyms
NFATC1; NFAT2; NFATC; Nuclear factor of activated T-cells; cytoplasmic 1; NF-ATc1; NFATc1; NFAT transcription complex cytosolic component; NF-ATc; NFATc
Documentation
User Guide for Antibodies (1077 KB)

Phospho-NFAT2 (Ser237) Antibody (YA1389) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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