Wnt3 Antibody (YA5324)
(Synonyms: Int 4; INT4; MGC131950; MGC138321; MGC138323; Proto-oncogene Int-4 homolog; Proto-oncogene Wnt-3; wingless type MMTV integration site family member 3; Wnt 3 proto oncogene protein; WNT 3 proto oncogene protein precursor; wnt3; WNT3_HUMAN.)Wnt3 Antibody (YA5324) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Wnt3.
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Host:
Mouse
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Application:
WB
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Reactivity :
Transfected
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:1000 |
Product Details
Wnt3 Antibody (YA5324) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Wnt3.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityTransfected
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Observed Molecular WeightObserved band size: 40 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Purified recombinant human Wnt3 protein fragments expressed in E.coli.
Transfected
affinity chromatography.
Non-conjugated
Unmodified
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Wnt3 is a secreted Wnt ligand linked to canonical Wnt/β-catenin signaling, a pathway that controls embryonic development and adult homeostasis[1]. Mechanistically, Wnt3 activity supports TCF/LEF-dependent transcription and connects ligand-receptor signaling to cell fate regulation[1]. In mouse embryos, Wnt3 expression precedes gastrulation and becomes restricted to the posterior epiblast, visceral endoderm, primitive streak, and mesoderm[2]. Wnt3- embryos fail to form a primitive streak, mesoderm, or node, establishing Wnt3 as a core regulator of vertebrate axis formation[2]. In limb development, ectodermal Wnt3/β-catenin signaling establishes and maintains the apical ectodermal ridge, linking Wnt3 to limb morphogenesis[3]. Human evidence further connects homozygous WNT3 mutation with tetra-amelia, supporting its early requirement in limb, craniofacial, and urogenital development[4]. Compared with Wnt3a, Wnt3 shows distinct activity in spinal cord neural precursors: Wnt3 transiently increases proliferation and enhances neurogenesis, whereas Wnt3a sustains proliferation[5]. For experimental applications, Wnt3/Wnt3a pathway studies can use canonical Wnt agonists, antagonists, or GSK-3β inhibitors to interrogate β-catenin-dependent proliferation, neurogenesis, and neurite outgrowth[1][5].
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Subcellular Localization
Secreted, extracellular space, extracellular matrix; Secreted
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Subunit
Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity (PubMed:26902720). The complex with AFM may represent the physiological form in body fluids (PubMed:26902720). Interacts with PORCN. Interacts with WLS (By similarity)
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SwissProt ID
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Synonyms
Int 4; INT4; MGC131950; MGC138321; MGC138323; Proto-oncogene Int-4 homolog; Proto-oncogene Wnt-3; wingless type MMTV integration site family member 3; Wnt 3 proto oncogene protein; WNT 3 proto oncogene protein precursor; wnt3; WNT3_HUMAN.
Documentation
References
[1]. MacDonald BT, et al. Wnt/beta-catenin signaling: components, mechanisms, and diseases. Dev Cell. 2009 Jul;17(1):9-26. [Content Brief]
[2]. Liu P, et al. Requirement for Wnt3 in vertebrate axis formation. Nat Genet. 1999 Aug;22(4):361-5. [Content Brief]
[3]. Barrow JR, et al. Ectodermal Wnt3/beta-catenin signaling is required for the establishment and maintenance of the apical ectodermal ridge. Genes Dev. 2003 Feb 1;17(3):394-409. [Content Brief]
[4]. Niemann S, et al. Homozygous WNT3 mutation causes tetra-amelia in a large consanguineous family. Am J Hum Genet. 2004 Mar;74(3):558-63. [Content Brief]
[5]. David MD, et al. Wnt-3a and Wnt-3 differently stimulate proliferation and neurogenesis of spinal neural precursors and promote neurite outgrowth by canonical signaling. J Neurosci Res. 2010 Nov 1;88(14):3011-23. [Content Brief]
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