alpha+beta Synuclein Antibody (YA6973)

Cat. No.: HY-P87290: Ficha de datos User Guide for Antibodies
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alpha+beta Synuclein Antibody (YA6973) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to alpha+beta Synuclein.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

alpha+beta Synuclein Antibody (YA6973) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to alpha+beta Synuclein.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Peso molecular

Predicted band size: 14 kDa;

Observed band size: 15 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P37840

Immunogen

Synthetic peptide within Human alpha+beta Synuclein aa 2-50 / 140.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-1:200
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:5000
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Pureza	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed:20798282, PubMed:26442590, PubMed:28288128, PubMed:30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590)

Subcellular Localization：Cytoplasm,Membrane,Nucleus,Synapse,Secreted,Cell projection, axon

Expression：
Tissue_Specificity: Highly expressed in presynaptic terminals in the central nervous system. Expressed principally in brain

Isoforms & Post-Translational Modification：P37840 has three isomers: P37840-1: 14460 Da (predicted); P37840-2: 11372 Da (predicted); P37840-3: 13109 Da (predicted).
Phosphorylated, predominantly on serine residues丨Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers丨Ubiquitinated丨Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure

Subunit：Soluble monomer

Synonyms

Alpha synuclein antibody; Beta synuclein antibody; NACP antibody; Non A beta component of AD amyloid antibody; PARK1 antibody; PARK4 antibody; PD1 antibody; SNCA antibody; SNCB antibody; Synuclein alpha antibody

Documentación

User Guide for Antibodies (1077 KB)