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BDNF Antibody(YA9955)

(Synonyms: BDNF; MGC34632; Abrineurin; ANON2; Brain Derived Neurotrophic Factor; Neurotrophin; BULN2)
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Based on 1 Customer Validation

BDNF Antibody(YA9955) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BDNF.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for BDNF Antibody(YA9955)
  • BDNF Antibody(YA9955) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-1:20000 1:50-1:500 1:50-1:200

Product Details

Description

BDNF Antibody(YA9955) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BDNF.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 15-45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 28 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human BDNF aa150-200.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for BDNF Antibody(YA9955)
    Western blot analysis was performed on protein extracts (25 μg) from Raji (lane 2), Jurkat (lane 3), and HepG2 (lane 4) using Proteasome 20S LMP7 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    BDNF is important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability[1].

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_Specificity: Detected in blood plasma and in saliva (at protein level). Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.

  • Isoforms & Post-Translational Modification

    BDNF has 5 isoforms, P23560-1: amino acid length is 247, molecular weight is 27818 Da (predicted); P23560-2: amino acid length is 255, molecular weight is 28872 Da (predicted); P23560-3: amino acid length is 262, molecular weight is 29817 Da (predicted); P23560-4: amino acid length is 329, molecular weight is 37120 Da (predicted); P23560-5: amino acid length is 276, molecular weight is 31116 Da (predicted).N-glycosylated and glycosulfated, contrary to mature BDNF

  • Subunit

    Monomers and homodimers.

  • SwissProt ID

    P23560

  • Gene ID
    627 [NCBI]
  • Synonyms

    BDNF; MGC34632; Abrineurin; ANON2; Brain Derived Neurotrophic Factor; Neurotrophin; BULN2

  • Research Field

    Neuroscience

References

[1]. Mowla SJ, et al. Biosynthesis and post-translational processing of the precursor to brain-derived neurotrophic factor. J Biol Chem. 2001 Apr 20;276(16):12660-6. [Content Brief]

BDNF Antibody(YA9955) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Raji (lane 2), Jurkat (lane 3), and HepG2 (lane 4) using Proteasome 20S LMP7 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • BDNF Antibody(YA9955) Product Image
    1/16
  • Experimental Validation Results for BDNF Antibody(YA9955)
  • BDNF Antibody(YA9955) Product Image
  • Experimental Validation Results for BDNF Antibody(YA9955)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Raji (lane 2), Jurkat (lane 3), and HepG2 (lane 4) using Proteasome 20S LMP7 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for BDNF Antibody(YA9955)
  • BDNF Antibody(YA9955) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from Raji (lane 2), Jurkat (lane 3), and HepG2 (lane 4) using Proteasome 20S LMP7 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • BDNF Antibody(YA9955) Product Image WB